■本研究旨在评估实时聚合酶链反应(PCR)在诊断慢性子宫内膜炎(CE)中的实用性,并将其结果与基于常规培养的诊断进行比较。
■对500例有异常出血等临床情况的患者进行回顾性分析,体外受精失败,复发性植入失败,复发性流产,和反复怀孕。在子宫内膜活检样本中评估了与CE相关的11种关键病原体的患病率。
■在我们的研究中,PCR鉴定出318例(63.6%)对11种病原体中的至少一种呈阳性,而基于培养的方法检测到115例(23%)。通过PCR检测到的主要病原体包括粪肠球菌(E。粪肠)(19%),大肠杆菌(E.大肠杆菌)(6.8%),金黄色葡萄球菌(S。金黄色葡萄球菌)(9%),人型支原体(5%),生殖支原体(6.2%),无乳链球菌(S.无乳)(4.2%),解脲脲原体(4%),非结核分枝杆菌(5.2%),结核分枝杆菌(1.2%),淋病奈瑟菌(0.6%),沙眼衣原体(2.4%)。标准培养方法确定粪肠球菌(10.8%),金黄色葡萄球菌(6.2%),大肠杆菌(3.8%),和无乳链球菌(2.2%)。
■DICE面板证明了自己是一个快速的,精确,和经济有效的诊断工具,用于检测CE中可培养和不可培养的子宫内膜病原体。展示优越性,分子方法胜过微生物培养,确保CE相关病原体的准确和灵敏检测,与组织学和宫腔镜检查结果无缝协调。
UNASSIGNED: This study aimed to assess the utility of real-time-polymerase chain reaction (PCR) for diagnosing chronic endometritis (CE) by targeting 11 prevalent pathogens and to compare the outcomes with conventional culture-based diagnosis.
UNASSIGNED: A retrospective analysis was conducted on 500 patients with clinical conditions such as abnormal bleeding, in vitro fertilization failure, recurrent implantation failure, recurrent miscarriage, and recurrent pregnancy loss. The prevalence of 11 key pathogens associated with CE was evaluated in endometrial biopsy samples.
UNASSIGNED: In our study, PCR identified 318 cases (63.6%) positive for at least one of the 11 investigated pathogens, while culture-based methods detected 115 cases (23%). Predominant pathogens detected by PCR included Enterococcus faecalis (E. faecalis) (19%), Escherichia coli (E. coli) (6.8%), Staphylococcus aureus (S. aureus) (9%), Mycoplasma hominis (5%), Mycoplasma genitalium (6.2%), Streptococcus agalactiae (S. agalactiae) (4.2%), Ureaplasma urealyticum (4%), nontuberculous Mycobacterium (5.2%), Mycobacterium tuberculosis (1.2%), Neisseria gonorrhoeae (0.6%), and Chlamydia trachomatis (2.4%). Standard culture methods identified E. faecalis (10.8%), S. aureus (6.2%), E. coli (3.8%), and S. agalactiae (2.2%).
UNASSIGNED: The DICE panel proves itself as a swift, precise, and cost-effective diagnostic tool for detecting both culturable and nonculturable endometrial pathogens in CE. Demonstrating superiority, the Molecular method outshines microbial culture, ensuring accurate and sensitive detection of CE-associated pathogens, harmonizing seamlessly with histology and hysteroscopy findings.