为了保持基因组的稳定性,生物体依赖于忠实的染色体分离,受不同遗传途径影响的过程,其中一些与有丝分裂没有直接联系。在这项研究中,我们开始探索一种由特征不足的基因代表的途径,SNO1,先前在有丝分裂保真基因的酵母敲除(YKO)文库的筛选中鉴定。我们发现,在sno1Δ突变体中增加有丝分裂错误率的致病因素不是Sno1蛋白的丢失,而是对邻近会聚基因的mRNA的扰动,CTF13,编码形成酵母动粒的必需成分。这是由在YKO文库中使用的卡那霉素抗性基因和转录终止子的组合影响CTF13mRNA水平和质量引起的。我们进一步提供了可能遭受这种人工制品的基因对的列表,这可能有助于YKO突变体的准确表型解释。
To maintain genome stability, organisms depend on faithful chromosome segregation, a process affected by diverse genetic pathways, some of which are not directly linked to mitosis. In this study, we set out to explore one such pathway represented by an undercharacterized gene, SNO1, identified previously in screens of the yeast knockout (YKO) library for mitotic fidelity genes. We found that the causative factor increasing mitotic error rate in the sno1Δ mutant is not loss of the Sno1 protein, but rather perturbation to the mRNA of the neighboring convergent gene, CTF13, encoding an essential component for forming the yeast kinetochore. This is caused by a combination of the Kanamycin resistance gene and the transcriptional terminator used in the YKO library affecting the CTF13 mRNA level and quality . We further provide a list of gene pairs potentially subjected to this artifact, which may be useful for accurate phenotypic interpretation of YKO mutants.