二维液相色谱(2D-LC)特别是全面的二维液相色谱(LC×LC),提供增加的峰值容量,与一维液相色谱相比,分辨率和选择性。人们普遍认为,当两个维度的分离机制完全正交时,该技术会产生最佳结果;然而,在两个维度中使用类似的分离机制已经越来越受欢迎,因为它有助于避免与流动相不相容性和柱效率差相关的困难。在两个维度(RPLC×RPLC)上使用反相优于其他分离机制的显着优势使其成为分离复杂样品的有前途的技术。这篇评论讨论了2D-LC方法开发中涉及在两个维度上使用RP的一些物理和实际考虑因素。此外,广泛的概述介绍了不同的应用,这些应用依赖于RPLC×RPLC和2D-LC与反相色谱柱组合来分离不同领域的复杂样品的成分,包括食品分析,天然产物分析,环境分析,蛋白质组学,脂质组学和代谢组学。
Two-dimensional liquid chromatography (2D-LC), and in particular comprehensive two-dimensional liquid chromatography (LC×LC), offers increased peak capacity, resolution and selectivity compared to one-dimensional liquid chromatography. It is commonly accepted that the technique produces the best results when the separation mechanisms in the two dimensions are completely orthogonal; however, the use of similar separation mechanisms in both dimensions has been gaining popularity as it helps avoid difficulties related to mobile phase incompatibility and poor column efficiency. The remarkable advantages of using reversed phase in both dimensions (RPLC×RPLC) over other separation mechanisms made it a promising technique in the separation of complex samples. This
review discusses some physical and practical considerations in method development for 2D-LC involving the use of RP in both dimensions. In addition, an extensive overview is presented of different applications that relied on RPLC×RPLC and 2D-LC with reversed phase column combinations to separate components of complex samples in different fields including food analysis, natural product analysis, environmental analysis, proteomics, lipidomics and metabolomics.