沉积和浮选技术等共同研究方法广泛用于研究猿猴胃肠道寄生虫。在自由范围内研究了四种具有已知人畜共患潜力的寄生虫,未提供的mandarles(Mandrillussphinx)种群:2个线虫(Necatoramicanus/食道。复合体和类圆线虫sp。)和2种原生动物(Balantidiumcoli和Entamoebacoli)。有不同的协同技术可用,但很少进行比较以评估其检索寄生虫的效率。在这项研究中,比较了4种不同的现场友好方法。一种沉降法和3种不同的麦克马斯特方法(使用糖,盐,和硫酸锌溶液)对从男女和所有年龄段的不同个体收集的47个粪便样本进行了处理。首先,我们表明McMaster浮选方法适用于检测并量化大型原生动物囊肿。第二,与其他3种方法相比,硫酸锌McMaster浮选可以回收更多的寄生虫类群。该方法进一步显示了检测每个所研究的寄生虫分类群的最高概率。总之,我们的结果表明,硫酸锌麦克马斯特浮选似乎是研究线虫和大型原生动物时使用的最佳技术。
Coproscopical methods like sedimentation and flotation techniques are widely used in the field for studying simian gastrointestinal parasites. Four parasites of known zoonotic potential were studied in a free-ranging, non-provisioned population of mandrills (Mandrillus sphinx): 2 nematodes (Necatoramericanus/Oesophagostomum sp. complex and Strongyloides sp.) and 2 protozoan species (Balantidium coli and Entamoeba coli). Different coproscopical techniques are available but they are rarely compared to evaluate their efficiency to retrieve parasites. In this
study 4 different field-friendly methods were compared. A sedimentation method and 3 different McMaster methods (using sugar, salt, and zinc sulphate solutions) were performed on 47 faecal samples collected from different individuals of both sexes and all ages. First, we show that McMaster flotation methods are appropriate to detect and thus quantify large protozoan cysts. Second, zinc sulphate McMaster flotation allows the retrieval of a higher number of parasite taxa compared to the other 3 methods. This method further shows the highest probability to detect each of the studied parasite taxa. Altogether our results show that zinc sulphate McMaster flotation appears to be the best technique to use when studying nematodes and large protozoa.