牛心包膜已被提议作为组织工程和生物假体重建的可用材料。在这项研究中,将牛心包制成支架,用于培养人脂肪干细胞(hADSC)和软骨分化。牛心包在10mMTris-HCl和0.15%SDS中处理,然后在0.1%戊二醛中交联。处理过的牛心包膜(tBP)的特征是微黄的薄膜,具有增强的拉伸强度和应变性能。膜在酶促条件下维持稳定性长达16天的孵育。结果证实,由于低细胞毒性和其支持hADSC的适当附着和增殖的能力,tBP作为hADSC的细胞友好支架。此外,在软骨形成诱导7天和14天后,在接种hADSC的tBP中存在细胞外基质蛋白聚糖的积累。7天后检测到COMP作为软骨形成的特异性标志物,而X-a1型胶原(Col10a1)表达稳定至第14天。然而,发现了aggrecan的次要表达。一起来看,这些结果表明tBP是hADSCs用于软骨组织工程的潜在支架。关键词:牛心包,脚手架,脂肪干细胞,软骨分化,软骨再生,隆鼻.
Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this
study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde. Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity and its ability to support an appropriate attachment and proliferation of hADSCs. Moreover, there was an accumulation of the extracellular matrix proteoglycan in tBP seeded with hADSCs after 7 and 14 days of chondrogenic induction. COMP as a specific marker of chondrogenesis was detected after 7 days, whereas type X-a1 collagen (Col10a1) expression was stable up to day 14. However, minor expression of aggrecan was found. Taken together, these results indicate that tBP is a potential scaffold for hADSCs for cartilage tissue engineering.Key words: Bovine pericardium, scaffold, adipose-derived stem cells, chondrogenic differentiation, cartilage regeneration, augmentation rhinoplasty.