苦瓜(MomordicacharantiaL.)是葫芦科的成员,长期以来因其丰富的生物活性成分或次生代谢产物而被用作食品和药物的来源。然而,大规模检测相对较少,identification,不同颜色苦瓜果皮中黄酮类化合物的定量研究。确定不同颜色苦瓜果皮中黄酮成分的多样性和特异性差异,三种彩色苦瓜在果皮中的代谢谱,深绿色(mo),浅绿色(lv),和白色(白),采用超高效液相色谱-串联质谱法进行分析。首先,证实绿色的不同色调是由叶绿素含量引起的。共有93种代谢物,包括90种黄酮类化合物和3种单宁,在当前的研究中检测到。这90个类黄酮包括三个异黄酮,九种二氢黄酮,七种黄烷醇,34黄酮醇,26类黄酮,四个查尔酮,五种类黄酮糖甙,和两种二氢黄酮醇.与莫相比,lv和bai都有21和25种不同的代谢物,分别,而lv和bai之间只有9种不同的代谢物。随着苦瓜颜色的加深,牡蛎素和异牡蛎素的相对含量增加。因此,有色苦瓜中的不同代谢产物主要参与黄酮醇的生物合成,类黄酮,和类黄酮.这项研究能够鉴定不同颜色苦瓜果皮的代谢差异。研究结果将有助于苦瓜新品种的优质选育,为其医药应用提供参考。
UNASSIGNED:在线版本包含补充材料,可在10.1007/s12298-022-01210-7获得。
Bitter gourd (Momordica charantia L.) is a member of Cucurbitaceae family and has long been used as a source of food and medicine for its rich bioactive components or secondary metabolites. However, there are relatively few large-scale detection, identification, and quantitative studies on flavonoids in the pericarp of bitter gourds of different colours. To determine the differences in the diversity and specificity of flavonoids in the pericarp of bitter gourd of different colours, the metabolic profiles in the pericarp of three coloured bitter gourd accessions, dark green (mo), pale green (lv), and white (bai), were analysed by ultra-performance liquid chromatography-tandem mass spectrometry. Priorly, it was confirmed that the different shades of green were caused by the content of chlorophyll. A total of 93 metabolites, including 90 flavonoids and three tannins, were detected in the current
study. These 90 flavonoids included three isoflavones, nine dihydroflavones, seven flavanols, 34 flavonols, 26 flavonoids, four chalcones, five flavonoid carbonosides, and two dihydroflavonols. Compared to mo, both lv and bai had 21 and 25 different metabolites, respectively, while there were only nine different metabolites between lv and bai. The relative contents of vitexin and isovitexin increased with the deeper colour of the bitter gourd. Thus, the different metabolites in coloured bitter gourds are mainly involved in the biosynthesis of flavonols, flavonoid carbonosides, and flavonoids. This
study enables identification of metabolic differences in the pericarp of bitter gourds of different colours. The results will be helpful for quality breeding of new bitter gourd varieties and shall provide a reference for their medical application.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12298-022-01210-7.