巴黎多叶树史密斯var。中国(法国。)Hara是一种药用植物,属于百合科。它的主要成分是parissaponins,具有良好的药用效果,如抗炎,抗肿瘤,等。通过人工定向调控提高香豆素的质量已成为满足医疗需求的实践,是一个新的研究热点。在本文中,多叶草植物用UVA处理,UVB,和UVC,以及PolyphyllinVI(PPVI)和PolyphyllinVII(PPVII)的含量,皂苷合成酶(角鲨烯合成酶,SS;环阿替诺醇合成酶,CAS;细胞色素P450,CYP450;和糖基转移酶,GT)活性,MDA,并对光合色素指标进行了测定和分析。结果表明,UVC处理4h后PPVII含量增加了32.43%(3.43mg/g),但其他组的PPVI和PPVII含量与CK(对照组)相比下降,并且在4h后没有恢复到原始水平。SS,CAS,CYP450和GT合酶通过紫外线处理在不同程度上被激活,分别,22.93%,10.83%,20.15%,和25.98%。其中,GT,作为最后的合成酶,对UVB(30分钟)和UVC(15分钟)的响应时间较短;与CK相比,差异是明显的。此外,紫外线具有胁迫作用,促进MDA含量的快速积累(增加17.66%,34.53%,和9.65%)和类胡萝卜素(与CK相比,在4小时内增加了7.58、5.60和7.76倍)。UVB和UVC辐射明显提高了叶绿素a含量(42.56%和35.45%),但是UVA没有,叶绿素b含量的变化没有明显的统计学差异。此外,PPVI和PPVII与SS呈负相关,CAS,类胡萝卜素,和MDA(p<0.05),并与CYP450,GT,和叶绿素a(p<0.05)。本研究为利用紫外光调控多叶紫菜次生代谢提供了理论依据,这对生产管理具有重要价值。
Paris polyphylla Smith var. Chinensis (Franch.) Hara is a medicinal plant that belongs to the Liliaceae family. Its main components are parissaponins, which have excellent medicinal effects such as anti-inflammatory, anti-tumor, etc. Improving the quality of parissaponins through artificial directional regulation has emerged as a practice to meet medical demand and is a new research hotspot. In this paper, P. polyphylla plants were treated with UVA, UVB, and UVC, and the contents of PolyPhyllin VI (PPVI) and PolyPhyllin VII (PPVII), saponin synthase (squalene synthase, SS; cycloartenol synthase, CAS; cytochrome P450, CYP450; and glycosyl transferases, GT) activity, MDA, and the photosynthetic pigment indexes were measured and analyzed. The results showed that PPVII content increased by 32.43% with UVC treatment after 4 h (3.43 mg/g), but the PPVI and PPVII contents in the other groups decreased compared with CK (control group) and they did not return to the original level after 4 h. SS, CAS, CYP450, and GT synthases were activated in varying degrees via UV treatment and increased, respectively, by 22.93%, 10.83%, 20.15%, and 25.98%. Among them, GT, as the last of the synthetases, had a shorter response time to UVB (30 min) and UVC (15 min); the difference was sensible compared with CK. Moreover, UV had a stressing effect and promoted the rapid accumulation of MDA content (increased 17.66%, 34.53%, and 9.65%) and carotenoid (increased 7.58, 5.60, and 7.76 times) within 4 h compared to CK. UVB and UVC radiation visibly improved chlorophyll a content (42.56% and 35.45%), but UVA did not, and the change in chlorophyll b content showed no overt statistical difference. In addition, PPVI and PPVII were negatively correlated with SS, CAS, carotenoids, and MDA (p < 0.05) and positively correlated with CYP450, GT, and chlorophyll a (p < 0.05). This
study provides a theoretical basis for using UV light to regulate secondary metabolism in P. polyphylla, which is of great value for production management.