人工细胞融合通常是研究生物学和医学中不同应用的有价值的工具。包括自然发展,免疫反应,癌症转移和治疗分子的产生。等离子细胞融合,一种通过金纳米粒子和共振飞秒脉冲辐射来融合邻近细胞的特定细胞标记技术,已被证明对此类应用有用,允许高细胞特异性和整体低毒性。尽管有这些优势,促成等离子体聚变的众多实验因素往往导致低等的聚变效率,需要重复的实验和广泛的校准协议,以达到最佳的结果。在这项工作中,我们提出了一项旨在提高等离子体细胞融合在融合效率和细胞活力方面的整体性能的研究。通过改变脉冲通量,纳米粒子浓度,孵化时间,和文化处理协议,我们证明了在整个培养物的照射区域中恶性上皮细胞高达100%的融合。我们还表明,一些较小的细胞可以保持存活长达几天。这些结果将使等离子体融合在许多需要特定的研究和应用中发挥关键作用,高效的细胞-细胞融合。
Artificial cell fusion often serves as a valuable tool for studying different applications in biology and medicine, including natural development, immune response, cancer metastasis and production of therapeutic molecules. Plasmonic cell fusion, a technique that uses specific cell labeling by gold nanoparticles and resonant femtosecond pulse irradiation for fusing neighboring cells, has been demonstrated useful for such applications, allowing high cell specificity and an overall low toxicity. Despite these advantages, the numerous experimental factors contributing to plasmonic fusion have often led to subpar fusion efficiencies, requiring repeated experiments and extensive calibration protocols for achieving optimal results. In this work we present a
study that aims to improve the overall performance of plasmonic cell fusion in terms of fusion efficiency and cell viability. By varying the pulse fluence, nanoparticle concentration, incubation times, and culture handling protocols, we demonstrate up to 100% fusion of malignant epithelial cells across the entire irradiated area of the culture. We also show that some of the smaller cells may stay viable for up to several days. The results would allow plasmonic fusion to play a key role in numerous studies and applications that require specific, high-efficiency cell-cell fusion.