未经批准:非小细胞肺癌(NSCLC)是全球、致命的肿瘤疾病,传统和靶向治疗被证明效果较差,随之而来的副作用;因此,来自草药的植物成分可能提供有效的替代品,应该进行癌症干预测试。原癌基因酪氨酸激酶Src(c-Src)和粘着斑激酶(FAK)的激活和过表达导致细胞增殖和侵袭。因此,在目前的调查中,进行了计算机分析,以确定植物成分在阻断活性位点从而抑制c-Src和FAK活化中的分子干预,这反过来可以控制NSCLC的进展。
UNASSIGNED:使用MolegroVirtualDocker进行了计算机分析,Molegro分子查看器,和ClusPro服务器用于配体-蛋白质和蛋白质-蛋白质相互作用研究。使用标准的植物化学试验对迷迭香水醇提取物进行了植物化学分析和抗氧化活性测定,高效薄层色谱,和2,2-二苯基-1-吡啶酰肼测定。在A549细胞系上使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑(MTT)测定法证实了提取物在阻止细胞增殖中的有效性。
UNASSIGNED:计算机模拟分析表明迷迭香酸与靶蛋白FAK和c-Src的活性位点有效结合,阻止他们的活动。MTT分析显示水醇提取物具有剂量依赖性的有效抗增殖活性。植物化学分析证实,该提取物富含植物成分,具有94.9%的抗氧化活性,因此可以有效地消除自由基并抑制细胞进展。
UNASSIGNED:在计算机和体外研究中证实,存在于水醇提取物中的植物成分可以有效阻断靶蛋白的活性位点,从而控制NSCLC细胞的细胞增殖。提示中药是治疗非小细胞肺癌的有效替代药物。
UNASSIGNED: Non small cell lung cancer (NSCLC) is a global, fatal oncological malady to which conventional and targeted therapies proved less effective with consequent side effects; hence, phytocomponents from herbal sources may provide potent alternative and should be tested for cancer intervention. Activation and overexpression of proto-oncogene tyrosine kinase Src (c-Src) and focal adhesion kinase (FAK) lead to cell proliferation and invasion. Hence, in the present investigation, in silico analysis was carried out to identify molecular intervention of phytocomponents in blocking the active site and thus inhibiting c-Src and FAK activation, which in turn could control progression of NSCLC.
UNASSIGNED: In silico analysis was carried out using Molegro Virtual Docker, Molegro Molecular Viewer, and ClusPro server for ligand-protein and protein-protein interaction
study. Phytochemical analysis and assay for antioxidant activity of hydroalcoholic extract of Rosmarinus officinalis L. were carried out using standard phytochemical tests, high-performance thin-layer chromatography, and 2, 2-diphenyl-1-picrylhydrazyl assay. Effectiveness of extract in arresting cell proliferation was confirmed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay on A549 cell line.
UNASSIGNED: In silico analysis indicated effective binding of rosmarinic acid to the active site of target proteins FAK and c-Src, blocking their activity. MTT assay revealed potent antiproliferative activity of hydroalcoholic extract which acted in dose-dependent manner. Phytochemical analysis confirmed that the extract was rich in phytocomponents and had antioxidant activity of 94.9%, which could therefore effectively eliminate free radicals and inhibit cell progression.
UNASSIGNED: In silico and in vitro studies confirmed that phytocomponents present in hydroalcoholic extract of R. officinalis L. could effectively block the active site of target proteins and thus controlled cell proliferation on NSCLC cells, suggesting herb as an effective alternative medicine for the treatment of NSCLC.