简介:伯氏疏螺旋体(s.l.),莱姆病的病原体,是欧洲最普遍的蜱传病原体,主要由蓖麻病毒传播。这个滴答也循环了密螺旋体,复发性发烧物种组的成员。目标:进行了一项纵向研究以评估疏螺旋体的患病率。在寻找蜱虫。壁虱疏螺旋体患病率与一些变量之间的关系,如壁虱发育阶段,采样面积,并对测量的蜱密度进行了评估;此外,对B.burgdorferis.l.的分布进行了分析,以确定螨的危险期。方法:每月在三个不同的生态区(海岸,高原,和山)在西班牙西北部进行了为期2年的研究。通过PCR检测疏螺旋体DNA,针对鞭毛蛋白(fla)基因。阳性样品还在rrfA-rrlB基因间间隔区和甘油磷酸二酯磷酸二酯酶(GlpQ)基因进行了表征。结果:在11.84%的蓖麻中检测到B.burgdorferis.l.DNA。确定了五种莱姆疏螺旋体(Borreliaafzelii,B.Burgdorferisensustricto,Galinii疏螺旋体,Lusitaniae,和贝氏螺旋体)。一种单一的复发性发烧物种(B.miyamotoi)检测到(0.85%)。探查Ixodesfrontalis,尖锐器,网状皮肤,和皮肤边缘产生负面结果。B.burgdorferis.l.的患病率。在雌性蜱和山区明显较高。此外,在整个研究中未检测到B.burgdorferis.l.患病率分布的季节性模式。结论:在调查I.ricinus时检测到明显的B.burgdorferis.l.流行表明有很高的螨虫风险,尤其是在山区。没有证据表明B.burgdorferis.l.的患病率与探查蜱的密度之间存在关系,也没有迹象表明壁虱患病率的季节性规律。
Introduction: Borrelia burgdorferi sensu lato (s.l.), the causative agent of Lyme borreliosis, is the most prevalent tick-borne pathogen in Europe, where it is mainly transmitted by Ixodes ricinus. This tick also circulates Borrelia miyamotoi, a member of the relapsing fever group of species. Objectives: A longitudinal
study was performed to assess the prevalence of Borrelia spp. in questing ticks. Relationships between Borrelia prevalence in ticks and some variables such as tick development stage, sampling area, and questing tick density were assessed; in addition, the distribution of B. burgdorferi s.l. was analyzed to establish the periods of acarological risk. Methods: Ticks were collected monthly by flagging in three different ecological areas (coast, plateau, and mountain) in north-western Spain during a 2-year
study. Borrelia DNA was detected by PCR, targeting the flagellin (fla) gene. Positive samples were also characterized at the rrfA-rrlB intergenic spacer region and the glycerophosphodiester phosphodiesterase (GlpQ) gene. Results: B. burgdorferi s.l. DNA was detected in 11.84% of I. ricinus. Five Lyme Borrelia species were identified (Borrelia afzelii, B. burgdorferi sensu stricto, Borrelia garinii, Borrelia lusitaniae, and Borrelia valaisiana). One single relapsing fever species (B. miyamotoi) was detected (0.85%). Questing Ixodes frontalis, Ixodes acuminatus, Dermacentor reticulatus, and Dermacentor marginatus yield negative results. The prevalence of B. burgdorferi s.l. was significantly higher in female ticks and in the mountain area. In addition, a seasonal pattern in the B. burgdorferi s.l. prevalence distribution throughout the
study was not detected. Conclusions: The detection of a noticeable prevalence of B. burgdorferi s.l. in questing I. ricinus suggests a high acarological risk, especially in mountain area. There is no evidence of a relationship between the prevalence of B. burgdorferi s.l. and the density of questing ticks, nor traces of a seasonal pattern in the values of prevalence in ticks.