钾(K)和磷(P)是植物发育所需的重要营养元素,但它是广泛存在的不溶性形式为植物的吸收。为了提高生产力,生物肥料在促进植物生长中起着至关重要的作用。我们目前的工作重点是从番茄栽培土壤的农业土壤中分离磷酸钾溶解细菌。用分光光度法估算久效磷的钾和磷酸盐溶解和降解。在13个分离株中,两个分离株被证明是最好的P和K增溶剂。对细菌分离株(SDKVG02和SDKVG04)进行了优化,以获得分离株对57.5mgL-1和15.07mgL-1的最大P和K溶解。盆栽实验使用固定在载体材料上的SDKVG02和04进行,泥炭是最佳载体,总平均绿克和鹰嘴豆长度为11.66±0.066622.22±0.0577。通过SDKVG02和SDKVG04,在80ppm的MCP下实现MCP降解百分比,具有75.8%和64.10%。此外,生产有机酸,如苹果酸,邻苯二甲酸,抗坏血酸,烟酸,根据16SrRNA基因测序,将分离株鉴定为血球肠杆菌-SDKVG-02,阴沟肠杆菌SDKVG-04。KSB-PSB分离株还表达N-固定活性,这通过计算机分析得到证明。值得强调的是SDKVG02和04将是有效的生物肥料,可用于提高土壤肥力和作物生产力以及土壤中久效磷的降解。
Potassium (K) and phosphorus (P) are the important macronutrients needed for the plant development, but it is widely present in an insoluble form for the plant\'s uptake. In order to increase the productivity, biofertilisers play crucial role in plant growth enhancement. Our present work focused to isolate potassium-phosphate solubilizing bacteria from the agricultural soil of tomato cultivated soil. Potassium and phosphate solubilization and degradation of monocrotophos was estimated spectrophotometrically. Out of thirteen isolates, two isolates proved to be the best P and K solubilizers. The bacterial isolates (SDKVG02 and SDKVG04) were optimized to obtain maximum P and K solubilization of 57.5 mg L-1 and 15.07 mg L-1 by the isolates. Pot experiments were conducted using SDKVG 02 and 04, immobilized on carrier materials, peat proving the best carrier with the total average green gram and chick pea length of 11.66 ± 0.0666 22.22 ± 0.0577. The MCP degradation percentage was achieved at 80 ppm of MCP with 75.8% and 64.10% by SDKVG 02 and SDKVG 04. Furthermore, production of organic acids such as malic acid, phthalic acid, ascorbic acid, nicotinic acid, and tartaric acid paves solubilization of P and K. The isolates were recognized based on 16S rRNA gene sequencing as Enterobacter hormaechei- SDKVG-02, Enterobacter cloacae SDKVG- 04. The KSB-PSB isolates also express N-fixing activity which is proved through In-silico analysis. It is worth to highlight SDKVG 02 and 04 would be potent biofertiliser exploited in increasing the soil fertility and crop productivity as well in degradation of monocrotophos present in the soil.