目的:经常遇到NFKBIA。然而,它对扩散的表达和相关性,入侵,和人类宫颈癌(CC)的迁移仍不清楚。本研究探讨了NFKBIA在CC进展中的作用和新机制。
方法:我们分析了NFKBIA在CC和癌旁正常组织中的表达,并探讨了其增殖。迁移,和通过用野生型NFKBIA质粒或NFKBIAsiRNA处理HeLa细胞的侵袭。随后评估了NFKBIA对上皮-间质转化(EMT)和β-catenin介导的靶基因转录的影响。
结果:NFKBIA在CC组织中的表达低于癌旁组织。在CC细胞增殖中发现NFKBIA过表达明显失调,入侵,和移民,这与敲低NFKBIA的效果不同。NFKBIA过表达促进磷酸化β-连环蛋白和E-钙黏着蛋白的表达。它抑制波形蛋白的表达,扭曲,以及β-连环蛋白的下游靶标,包括c-MYC,TCF-4和MMP14。相反,NFKBIA沉默升高c-MYC的表达,TCF-4和MMP14促进了HeLa细胞的EMT。内源性和外源性NFKBIA均与β-catenin相互作用。此外,β-catenin过表达阻止了NFKBIA对增殖的影响,迁移,和HeLa细胞的入侵。通过在体内过度表达NFKBIA,肿瘤的体积和大小明显减小,而小鼠体重无明显变化。
结论:通过抑制β-catenin介导的转录,作为肿瘤抑制因子的NFKBIA可能被引入作为用于治疗靶向CC的新型抗转移剂。
OBJECTIVE: NFKBIA is frequently encountered. However, its expression and relevance of the proliferation, invasion, and migration in human cervical cancer (CC) remain unclear. The role and novel mechanism of NFKBIA in CC progression were investigated in this
study.
METHODS: We analyzed the expression of NFKBIA in CC and adjacent normal tissues and explored the proliferation, migration, and invasion of HeLa cells by treating with either wild-type NFKBIA plasmid or NFKBIA siRNA. Effect of NFKBIA on the epithelial-mesenchymal transition (EMT) and the β-catenin-mediated transcription of target genes were evaluated subsequently.
RESULTS: NFKBIA expression was lower in CC tissues than that of adjacent tissues. An obvious dysregulation of NFKBIA overexpression was revealed in CC cell proliferation, invasion, and migration, which differed from the effect of knockdown NFKBIA. NFKBIA overexpression facilitated the expression of both phosphorylated β-catenin and E-cadherin protein. It inhibited the expression of vimentin, TWIST, as well as downstream targets of β-catenin including c-MYC, TCF-4 and MMP14. Conversely, NFKBIA silencing elevated the expression of c-MYC, TCF-4, and MMP14, and promoted the EMT in HeLa cells. Both endogenous and exogenous NFKBIA interacted with β-catenin. Moreover, β-catenin overexpression stemmed effects of NFKBIA on the proliferation, migration, and invasion of HeLa cells. By overexpressing NFKBIA in vivo, the volume and size of tumors were notably decreased, while no obvious alteration was found in mice body weight.
CONCLUSIONS: By inhibiting β-catenin-mediated transcription, NFKBIA functioning as a tumor suppressor might be introduced as a novel anti-metastatic agent for the treatment of targeted CC.