地下水细菌污染一直是一个值得关注的生态问题。在这项研究中,分析了在宿主-病原体相互作用过程中具有不同鞭毛表型的肠沙门氏菌在多孔介质中的运输和沉积行为。在具有鞭毛运动性（野生型）或没有运动性（ΔmotAB）的不同菌株上进行了柱运输实验和改良的移动-固定模型，无鞭毛（ΔflgKL），甲基化和未甲基化鞭毛蛋白（ΔfliB），和不同的鞭毛阶段(FlyCON，fljBON)。结果表明，由于鞭毛具有移动和附着于表面的生物学优势，鞭毛运动可以促进细菌的运输和沉积。我们还发现，与ΔflgKL菌株相比，ΔmotAB菌株的保留率更高，因此非运动鞭毛的存在改善了细菌粘附。这表明细菌鞭毛和运动性都对沙质多孔介质中的细菌沉积具有促进作用。鞭毛相会影响细菌的运动；flagella菌株比fljBON菌株更快地通过色谱柱。此外，发现鞭毛甲基化有利于细菌的运输和沉积。总的来说,鞭毛修饰在环境条件下以不同方式影响肠道沙门氏菌的运输和沉积行为。
Bacterial contamination of groundwater has always been an ecological problem worthy of attention. In this study, Salmonella enterica serovar Typhimurium with different flagellar phenotypes mainly characterized during host-pathogen interaction were analyzed for their transport and deposition behavior in porous media. Column transport experiments and a modified mobile-immobile model were applicated on different strains with flagellar motility (wild-type) or without motility (ΔmotAB), without flagella (ΔflgKL), methylated and unmethylated flagellin (ΔfliB), and different flagella phases (fliCON, fljBON). Results showed that flagella motility could promote bacterial transport and deposition due to their biological advantages of moving and attaching to surfaces. We also found that the presence of non-motile flagella improved bacterial adhesion according to a higher retention rate of the ΔmotAB strain compared to the ΔflgKL strain. This indicated that bacteria flagella and motility both had promoting effects on bacterial deposition in sandy porous media. Flagella phases influenced the bacterial movement; the fliCON strain went faster through the column than the fljBON strain. Moreover, flagella methylation was found to favor bacterial transport and deposition. Overall, flagellar modifications affect Salmonella enterica serovar Typhimurium transport and deposition behavior in different ways in environmental conditions.
