神经内分泌肿瘤(NENs)是临床行为异质性的罕见肿瘤。据报道,人类微生物群的变化与不同实体瘤的致癌作用有关。然而,很少有研究探讨微生物群在NEN中的作用。我们在此旨在评估神经内分泌肿瘤组织中细菌浸润的存在。为了评估细菌的存在,通过荧光原位杂交和共聚焦显微镜评估来自胰腺NEN(pan-NEN)的20个样本和来自肠NEN(I-NEN)的20个样本。人口统计数据,术前调查,手术发现,病理诊断,后续行动,和生存数据进行了评估。在I-NEN中,15/20(75%)标本中检测到细菌,微生物分布具有高度变异性。在八个病人中,观察到微生物的高度浸润。在pan-NEN中,18/20(90%)显示微生物浸润,具有均匀的微生物分布。在血管附近观察到pan-NEN中的细菌定位。在10/20pan-NEN(50%)中报道了与非肿瘤组织相比,肿瘤标本中的细菌浸润更高。根据年龄,平均细菌计数没有观察到显著差异,性别,ki67%,site,肿瘤分期。平均细菌计数不能预测疾病特异性存活。这项初步研究表明,在NEN微环境中存在重要的微生物群。需要进一步的研究来调查NEN中微生物群的潜在病因或临床作用。
Neuroendocrine neoplasms (NENs) are rare neoplasms with heterogeneous clinical behavior. Alteration in human microbiota was reported in association with carcinogenesis in different solid tumors. However, few studies addressed the role of microbiota in NEN. We here aimed at evaluating the presence of bacterial infiltration in neuroendocrine tumoral tissue. To assess the presence of bacteria, 20 specimens from pancreatic NEN (pan-NEN) and 20 from intestinal NEN (I-NEN) were evaluated through Fluorescent In situ Hybridization and confocal microscopy. Demographic data, pre-operative investigations, operative findings, pathological diagnosis, follow-up, and survival data were evaluated. Among I-NEN, bacteria were detected in 15/20 (75%) specimens, with high variability in microbial distribution. In eight patients, a high infiltration of microorganisms was observed. Among pan-NEN, 18/20 (90%) showed microorganisms\' infiltration, with a homogeneous microbial distribution. Bacterial localization in pan-NEN was observed in the proximity of blood vessels. A higher bacterial infiltration in the tumoral specimen as compared with non-tumoral tissue was reported in 10/20 pan-NEN (50%). No significant differences were observed in mean bacterial count according to age, sex, ki67%, site, tumor stage. Mean bacterial count did not result to be a predictor of disease-specific survival. This preliminary
study demonstrates the presence of a significant microbiota in the NEN microenvironment. Further research is needed to investigate the potential etiological or clinical role of microbiota in NEN.