Rhizosphere bacteria can have wide-ranging effects on their host plants, influencing plant biochemical and structural characteristics, and overall productivity. The implications of plant-microbe interactions provides an opportunity to interfere agriculture ecosystem with exogenous regulation of soil microbial community. Therefore, how to efficiently predict soil bacterial community at low cost is becoming a practical demand. Here, we hypothesize that foliar spectral traits can predict the diversity of bacterial community in orchard ecosystem. We tested this hypothesis by studying the ecological linkages between foliar spectral traits and soil bacterial community in a peach orchard in Yanqing, Beijing in 2020. Foliar spectral indexes were strongly correlated with alpha bacterial diversity and abundant genera that can promote soil nutrient conversion and utilization, such as Blastococcus, Solirubrobacter, and Sphingomonas at fruit mature stage. Certain unidentified or relative abundance <1% genera were also associated with foliar spectral traits. We selected specific indicators (photochemical reflectance index, normalized difference vegetable index, greenness index, and optimized soil-adjusted vegetation index) of foliar spectral indexes, alpha and beta diversities of bacterial community, and quantified the relations between foliar spectral traits and belowground bacterial community via SEM. The results of this study indicated that foliar spectral traits could powerfully predict belowground bacterial diversity. Characterizing plant attributes with easy-accessed foliar spectral indexes provides a new thinking in untangling the complex plant-microbe relationship, which could better cope with the decreased functional attributes (physiological, ecological, and productive traits) in orchard ecosystem.

Major hallmarks of functional loss, loss of metabolic and musculoskeletal health and (multi)morbidity with aging are associated with sleep disturbances. With poor sleep shifts in gut microbial composition commonly manifest, which could mediate the pro-inflammatory state between sleep disturbances and sarcopenia. This systematic review presents the recent evidence on how sleep disturbances throughout the lifespan associate with and contribute to gut microbial composition changes, proposing a mechanism to understand the etiology of sarcopenia through sleep disturbances. The relationship between disturbed sleep and clinically relevant gut microbiota composition on health aspects of aging is discussed. A search was performed in PubMed, Cochrane Library, Scopus, Web of Science using keywords including (microbio* OR microflora) AND (sleep OR sleep disorder). Six cross-sectional population-based studies and five experimental clinical trials investigating healthy individuals with ages ranging from 4 to 71 were included. The cross-sectional studies reported similarities in associations with sleep disturbance and gut microbial diversity. In older adults, shorter sleep duration is associated with an increase in pro-inflammatory bacteria whereas increasing sleep quality is positively associated with an increase of beneficial Verrucomicrobia and Lentisphaerae phyla. In young adults, the effect of sleep disruption on gut microbiome composition, specifically the ratio of beneficial Firmicutes over Bacteroidetes phyla, remains contradictory and unclear. The findings of this review warrant further research in the modulation of the gut microbiome linking poor sleep with muscle-catabolic consequences throughout the lifespan.

Microplastics (MPs), as new pollutants in agroecosystems, have already attracted widespread attention from scientists. However, our understanding of MP geographic distribution and its influencing factors across spatial scales remains poor. Here, a regional-scale field investigation was conducted to assess the distribution characteristic of MPs in five major rice-growing regions of China, and we explored the roles of biological and abiotic factors, especially stoichiometry and microbial influences on MP distribution. MPs were observed in all sampling sites, averaging 6,390 ± 2,031 items⋅kg-1. Sizes less than 0.5 mm and black and transparent MPs dominated. Fiber, classified as one of the MP shapes, occurred most frequently. MP community analysis, firstly used in paddy soil, revealed more black MPs abundance in Henan (HE), more rayon, blue, and other colors MPs in Hunan (HN), more transparent MPs in Tianjing (TJ), and more PE MPs in Heilongjiang (DB). Higher MP community diversity was found in most south paddy soils of this study, due to a broader range of sources. C/N showed a positive relationship with pellet-shaped MP abundance and MPs of size between 2 and 5 mm (P < 0.05). Chao1 index of soil microbial communities was positively correlated with the MP abundance, MPs of size less than 0.5 mm, and fiber abundance. The minimum temperature was positively correlated with MP abundance (P < 0.05), implying the potential effects of the freeze-thaw process might exist. The regression analysis highlighted the important role of population quantity in determining MP abundance (R = 0.421, P = 0.02). This study confirmed the wide distribution of MPs in different soil depths of paddy lands in China and demonstrated that its distribution was influenced by population quantity and environmental variables, such as microbiology. These findings could provide a basis for the toxicological behavior of MPs and the potential risk to human health.

Microbial communities often possess enormous diversity, raising questions about whether this diversity drives ecosystem functioning, especially the influence of diversity on soil decomposition and respiration. Although functional redundancy is widely observed in soil microorganisms, evidence that species occupy distinct metabolic niches has also emerged. In this paper, we found that apart from the environmental variables, increases in microbial diversity, notably bacterial diversity, lead to an increase in soil C emissions. This was demonstrated using structural equation modelling (SEM), linking soil respiration with naturally differing levels of soil physio-chemical properties, vegetation coverage, and microbial diversity after fire disturbance. Our SEMs also revealed that models including bacterial diversity explained more variation of soil CO2 emissions (about 45%) than fungal diversity (about 38%). A possible explanation of this discrepancy is that fungi are more multifunctional than bacteria and, therefore, an increase in fungal diversity does not necessarily change soil respiration. Further analysis on functional gene structure suggested that bacterial and fungal diversities mainly explain the potential decomposition of recalcitrant C compare with that of labile C. Overall, by incorporating microbial diversity and the environmental variables, the predictive power of models on soil C emission was significantly improved, indicating microbial diversity is crucial for predicting ecosystem functions.

Tanneries are an important industrial sector in Ethiopia; consequently, gaps in wastewater treatment process performance need to be identified as the country increases its emphasis on compliance. A case study was conducted to evaluate physicochemical and microbial water quality at a tannery near Addis Ababa. The treatment process was designed for the following: sulfide oxidation; biological oxygen demand reduction; and chromium removal. While some of Ethiopia\'s standards for industrial wastewater treatment were met through treatment, effluent COD, sulfide, total nitrogen, and total chromium guidelines were not. 16S rRNA gene analysis was used to evaluate the microbial community composition across the treatment train. The results show that common ruminant phyla were dominant throughout, with Firmicutes and Bacteroidetes comprising 77% to 82% relative abundance. The Firmicutes Clostridium increased consistently in relative abundance with treatment, comprising 39% to 61% of the total bacterial community in the effluent. Improved treatment is needed to meet environmental and public health goals. PRACTITIONER POINTS: Case Study of tannery wastewater treatment in Ethiopia shows ineffective treatment of chemical pollutants. Microbiological pollutants from tannery wastewater systems can introduce agents of importance to public health The microbiological composition of tannery influent, mixed liquor and effluent contains mostly four bacterial phyla lead by Firmicutes. Most pathogenic bacterial genera found in the tannery wastewater treatment system became a decreasing percentage of the total population. Clostridium comprises up to 61% of the effluent bacterial population and deserves further evaluation to better understand the consequences of its dominance.

OBJECTIVE: Aerobic vaginitis (AV) has drawn increasing attention because of its threat to women\'s reproductive health and pregnancy. However, little is known about the overall structure of vaginal bacterial communities in women with AV.
METHODS: The diversity of vaginal microbiota was evaluated by amplicon sequencing targeting the 16S rRNA V4 region. Routine laboratory tests, including cultivation, were used.
RESULTS: Firmicutes (mainly Lactobacillus crispatus and L. iners) were dominant in healthy women (n = 160), while Actinobacteria and Bacteroidetes were strongly associated with AV (n = 80). The onset of AV was marked by a striking decline in L. crispatus and an increase in multiple aerobes, including Streptococcus agalactiae, S. anginosus, etc. The overall drug resistance level of gram-positive bacteria against erythromycin and clindamycin was high, and the overall drug resistance level of gram-negative bacteria against ampicillin was high.
CONCLUSIONS: Multiple aerobes and facultative anaerobes were involved in vaginal dysbiosis, which was associated with decreasing L. crispatus levels. Probiotics containing L. crispatus may be potential supplementary agents.

Anthropogenic activities can cause serious negative effects on ecosystems. Despite the ecological significance of bacterial communities, the integrated biogeography of planktonic and sedimentary bacterial communities in response to anthropogenic activities is not adequately understood. Here, we examined environmental parameters and the composition of planktonic and sedimentary bacteria in the Yulin River, a tributary of Three Gorges Reservoir, in response to changes in land use and dam construction. The results revealed that human-induced land use changes enhanced the nutrient concentrations in surface water and dam construction reduced the content of carbon and nitrogen in immediately downstream sediments. Intensified human-dominated land use showed a slight impact on sedimentary bacterial communities but largely reduced the diversity of planktonic bacterial communities. Moreover, human-induced land use changes increased the abundance of genes associated with denitrification, nitrification, and anammox in planktonic bacterial communities by 19.04%, 32.40% and 30.45%, respectively. In dam construction regions, the diversity and nutrient-related metabolic activity of sedimentary bacterial communities immediately downstream of the dam were decreased, whereas these changes were not observed in planktonic bacterial communities. Additionally, bacterial community composition was significantly related to nutrient concentrations variability and followed a distance-decay pattern. Furthermore, environmental effects explained more of the variation in planktonic bacterial community composition as compared with spatial effects did, whereas, sedimentary bacterial communities were more closely related to spatial effects. Our results demonstrated the distinct responses of planktonic and sedimentary bacterial communities to anthropogenic activities, and offered new insight for understanding their potential ecological influence on rivers.