在涉及动物的研究文章中发表准确和详细的方法描述对于健康科学家准确解释已发表的数据至关重要,评估结果并重复发现。实验设计的关键方面报告不足可能会降低研究的影响,并可能成为研究结果翻译的障碍。动物使用的报告必须尽可能全面,以便利用每一项研究和使用的每只动物。动物模型对于了解和评估查加斯病病理的新化疗候选药物至关重要。一种广泛的寄生虫病,目前可用的治疗方法很少。进行了系统评价,以将ARRIVE指南建议与新的抗克氏锥虫化合物的临床前研究出版物中提供的信息进行比较。共审查了83份出版物。在到达指引之前,69%的出版物没有报告任何宏观环境信息,相比之下,ARRIVE出版后为57%。在评估微环境信息报告时观察到类似的比例(56%与61%)。此外,在ARRIVE指南发布之前,只有13%的论文描述了动物性别,只有18%的人指定了微生物状态,13%的人报告了随机治疗分配,在其他基本信息缺失或不完整中。不幸的是,ARRIVE指南的发布似乎没有提高报告质量,与ARRIVE出版之前出现的论文相比。我们的结果表明,科学界非常需要改进动物使用描述,采用的动物模型,透明的报告和实验设计,以促进其转移和应用到受影响的人口。完全符合ARRIVE指南,或类似的动物研究报告指南,这将是一个很好的开端。
Publication of accurate and detailed descriptions of methods in research articles involving animals is essential for health scientists to accurately interpret published data, evaluate results and replicate findings. Inadequate reporting of key aspects of experimental design may reduce the impact of studies and could act as a barrier to translation of research findings. Reporting of animal use must be as comprehensive as possible in order to take advantage of every study and every animal used. Animal models are essential to understanding and assessing new chemotherapy candidates for Chagas disease pathology, a widespread parasitic disease with few treatment options currently available. A systematic review was carried out to compare ARRIVE
guidelines recommendations with information provided in publications of preclinical studies for new anti-Trypanosoma cruzi compounds. A total of 83 publications were reviewed. Before ARRIVE
guidelines, 69% of publications failed to report any macroenvironment information, compared to 57% after ARRIVE publication. Similar proportions were observed when evaluating reporting of microenvironmental information (56% vs. 61%). Also, before ARRIVE
guidelines publication, only 13% of papers described animal gender, only 18% specified microbiological status and 13% reported randomized treatment assignment, among other essential information missing or incomplete. Unfortunately, publication of ARRIVE
guidelines did not seem to enhance reporting quality, compared to papers appeared before ARRIVE publication. Our results suggest that there is a strong need for the scientific community to improve animal use description, animal models employed, transparent reporting and experiment design to facilitate its transfer and application to the affected human population. Full compliance with ARRIVE
guidelines, or similar animal research reporting
guidelines, would be an excellent start in this direction.