戊型肝炎是一种与肝脏急性炎症相关的疾病。它与几种失调的代谢途径和几种代谢物浓度的改变有关。然而,通常缺乏对代谢物和脂质变化的纵向分析。这项研究调查了与年龄和性别相似的健康对照相比,急性戊型肝炎男性血清中代谢物和脂质水平随时间的变化。使用质谱法对从14例急性戊型肝炎患者中依次采样的65份血清和来自5例对照的25份血清样品进行各种代谢物和脂质水平的非目标测量。对于戊型肝炎病毒(HEV)组,在3天的不同时间内确定了代谢物和脂质强度的时间变化。在碳水化合物代谢中,葡萄糖水平,与健康对照组相比,HEV感染者的果糖1-6-二磷酸和核酮糖-5-磷酸增加。HEV感染与肌苷水平降低显著相关,鸟苷,嘌呤代谢和胸腺嘧啶中的腺苷和尿酸,嘧啶代谢中的尿嘧啶和β-氨基异丁酸。谷氨酸,HEV组的丙氨酸和缬氨酸水平明显低于健康个体。酪氨酸代谢的匀浆和丝氨酸代谢的胱硫醚增加,而HEV组的色氨酸代谢下降。胆汁酸生物合成的代谢物,尿素循环(精氨酸和瓜氨酸)和氨再循环(尿酸)发生了显着改变。辅酶,泛酸和吡哆醛,和共同因素,硫胺素和FAD,在HEV组中升高。酰基肉碱,鞘磷脂,磷脂酰胆碱(PC),磷脂酰乙醇胺(PE),HEV组的lysoPC和lysoPE趋于较低。总之,急性戊型肝炎与代谢产物和血脂改变有关,显着增加碳水化合物的分解代谢,嘌呤/嘧啶和氨基酸,并降低了几种甘油磷脂的水平。
Hepatitis E is a disease associated with acute inflammation of the liver. It is related to several dysregulated metabolic pathways and alterations in the concentration of several metabolites. However, longitudinal analysis of the alterations in metabolites and lipids is generally lacking. This
study investigated the changes in levels of metabolites and lipids over time in sera from men with acute hepatitis E compared to healthy controls similar in age and gender. Untargeted measurement of levels of various metabolites and lipids was done using mass spectrometry on 65 sera sequentially sampled from 14 patients with acute hepatitis E and 25 serum samples from five controls. Temporal changes in intensities of metabolites and lipids were determined over different times at 3-day periods for the hepatitis E virus (HEV) group. In carbohydrate metabolism, glucose levels, fructose 1-6-bisphosphate and ribulose-5-phosphate were increased in the HEV-infected persons compared to the healthy controls. HEV infection is significantly associated with decreased levels of inosine, guanosine, adenosine and urate in purine metabolism and thymine, uracil and β-aminoisobutyrate in pyrimidine metabolism. Glutamate, alanine and valine levels were significantly lower in the HEV group than in healthy individuals. Homogentisate of tyrosine metabolism and cystathionine of serine metabolism were increased, whereas kynurenate of tryptophan metabolism decreased in the HEV group. Metabolites of the bile acid biosynthesis, urea cycle (arginine and citrulline) and ammonia recycling (urocanate) were significantly altered. Co-enzymes, pantothenate and pyridoxal, and co-factors, lipoamide and FAD, were elevated in the HEV group. The acylcarnitines, sphingomyelins, phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysoPC and lysoPE tended to be lower in the HEV group. In conclusion, acute hepatitis E is associated with altered metabolite and lipid profiles, significantly increased catabolism of carbohydrates, purines/pyrimidines and amino acids, and decreased levels of several glycerophospholipids.