炎症小体最近与几种慢性炎症性疾病的发病机理有关。比如糖尿病和肥胖症。这项荟萃分析的目的是研究NLRP3炎性体在肥胖和多囊卵巢综合征(PCOS)中的可能作用。对电子数据库进行了全面搜索,以确定与非肥胖对照相比,研究肥胖个体脂肪组织和/或血液中NLRP3相关成分(Caspase1,ASC和IL-1β)的研究。另一项研究是在PCOS女性和动物模型中研究NLRP3。搜索的数据库包括Medline,EMBASE,科克伦图书馆,PubMed,Clinicaltrials.gov,欧盟临床试验登记册和世卫组织国际临床试验登记册。使用改良的纽卡斯尔-渥太华量表评估纳入文章的质量和偏倚风险。使用RevMan软件提取并汇集数据以计算标准化均差(SMD)和95%置信区间(CI)。12项符合条件的研究纳入肥胖系统评价,9项纳入PCOS评价。在肥胖研究中,9人(n=270)被纳入荟萃分析,与非肥胖(n=84)参与者相比,肥胖(n=186)参与者的脂肪组织NLRP3基因表达明显更高(SMD1.07;95%CI,0.27,1.87).来自四项研究的脂肪组织IL-1β数据的汇总分析显示,88名肥胖参与者的脂肪组织中IL-1β基因表达水平明显高于39名非肥胖对照组(SMD0.56;95%CI,0.13,0.99)。来自四项研究的脂肪组织ASC数据的荟萃分析显示,肥胖(n=109)与非肥胖(n=42)个体的水平显着更高(SMD0.91,95%CI,0.30,1.52)。在9篇PCOS文章中,3个是人(n=185),6个是利用PCOS大鼠/小鼠模型的动物研究.除一篇文章外,所有研究均一致显示NLRP3及其成分在PCOS女性和动物模型中上调。总之,肥胖和PCOS似乎与NLRP3炎性体成分表达上调有关。需要进一步的研究来验证这些发现,并阐明NLRP3在肥胖和PCOS中的作用。
Inflammasomes have recently been implicated in the pathogenesis of several chronic inflammatory disorders, such as diabetes and obesity. The aim of this meta-analysis was to investigate the possible role of the NLRP3 inflammasome in obesity and polycystic ovarian syndrome (PCOS). A comprehensive search of electronic databases was conducted to identify studies investigating NLRP3 its related components (Caspase 1,
ASC and IL-1β) in adipose tissue and/or blood from obese individuals compared to non-obese controls. Another search was conducted for studies investigating NLRP3 in PCOS women and animal models. The ssearched databases included Medline, EMBASE, Cochrane Library, PubMed, Clinicaltrials.gov, the EU Clinical Trials Register and the WHO International Clinical Trials Register. The quality and risk of bias for the included articles were assessed using the modified Newcastle-Ottawa scale. Data were extracted and pooled using RevMan software for the calculation of the standardized mean difference (SMD) and 95% confidence interval (CI). Twelve eligible studies were included in the obesity systematic
review and nine in the PCOS
review. Of the obesity studies, nine (n = 270) were included in the meta-analysis, which showed a significantly higher adipose tissue NLRP3 gene expression in obese (n = 186) versus non-obese (n = 84) participants (SMD 1.07; 95% CI, 0.27, 1.87). Pooled analysis of adipose tissue IL-1β data from four studies showed significantly higher IL-1β gene expression levels in adipose tissue from 88 obese participants versus 39 non-obese controls (SMD 0.56; 95% CI, 0.13, 0.99). Meta-analysis of adipose tissue
ASC data from four studies showed a significantly higher level in obese (n = 109) versus non-obese (n = 42) individuals (SMD 0.91, 95% CI, 0.30, 1.52). Of the nine PCOS articles, three were human (n = 185) and six were animal studies utilizing PCOS rat/mouse models. All studies apart from one article consistently showed upregulated NLRP3 and its components in PCOS women and animal models. In conclusion, obesity and PCOS seem to be associated with upregulated expression of NLRP3 inflammasome components. Further research is required to validate these findings and to elucidate the role of NLRP3 in obesity and PCOS.