在这份手稿中,生长和退火的锶掺杂的氧化镍纳米颗粒(SrNiONPs)是使用沉淀法以硝酸镍和硝酸锶为前驱剂,柠檬酸三钠合成的。各种表征技术,包括X射线衍射图(XRD),扫描电子显微镜(SEM),透射电子显微镜(TEM),傅里叶变换红外光谱(FTIR),UV-可见光,和zetasizer,被用来彻底检查样本。XRD图(21nm)用于计算尺寸,阶段,和材料的结晶度(SrNiONPs)。除了表征,测试该材料在肺癌细胞(A549)中的细胞毒性。使用[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物](MTT)和中性红摄取(NRU)测定进行A549细胞中的活力测试,SrNiONP浓度范围为1至100μg/mL。根据MTT和NRU数据,毒性研究是剂量依赖性的。SrNiONPs还增加了活性氧(ROS),并参与了细胞凋亡(A549细胞)。此外,定量PCR(qPCR)数据显示,凋亡基因的mRNA水平标记如p53,bax,caspase-3上调,而Bcl-2,一种抗凋亡基因,被下调。因此,细胞凋亡是由p53、bax、caspase3和bcl-2途径,暗示SrNiONPs介导其毒性的潜在机制。
In this manuscript, the grown and annealed strontium-doped nickel oxide nanoparticles (SrNiONPs) were synthesized using a precipitation method with nickel nitrate and strontium nitrate as precursor agents with trisodium citrate. Various characterization techniques, including X-ray diffraction pattern (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), UV-visible, and zeta sizer, were used to thoroughly examine the samples. The XRD pattern (21 nm) was used to calculate the size, phases, and crystallinity of the material (SrNiONPs). In addition to characterization, the material was tested for cytotoxicity in lung cancer cells (
A549). The viability test in
A549 cells was performed using [3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide] (MTT) and Neutral Red Uptake (NRU) assay with SrNiONPs concentration ranging from 1 to 100 μg/mL. According to the MTT and NRU data, the toxicity studies are dose-dependent. SrNiONPs also increased reactive oxygen species (ROS) and were involved in apoptosis (
A549 cells). Furthermore, quantitative PCR (qPCR) data revealed that the mRNA levels of apoptotic genes marker like p53, bax, and caspase-3 were upregulated, whereas bcl-2, an anti-apoptotic gene, was downregulated. As a result, apoptosis was mediated by the p53, bax, caspase3, and bcl-2 pathways, implying a potential mechanism by which SrNiONPs mediate their toxicity.