在核和线粒体DNA中,8-羟基-2'-脱氧鸟苷(8-OHdG)是活性氧(ROSs)病变的主要形式之一,通常用作氧化应激的生物标志物。研究表明,不同的纳米材料可以通过ROSs对人体产生毒性。所以,这项研究将回顾有关纳米材料职业暴露引起的DNA氧化损伤的研究,使用8-OHdG生物标志物。系统评价根据Cochrane系统评价指南进行管理。文献检索在科学数据库中进行,主要术语为“生物标志物”,\"\"生物标记,与“职业暴露”和“纳米材料”相结合。“截至2020年12月,纳米材料职业暴露领域的所有论文都包括在内。为了评估研究的质量和偏见,等级方法(建议的等级,评估,发展,和评估)被使用。主要完成了二十六项研究。通过考虑纳入标准,总共选择了8篇文章。大多数研究被归类为中等质量研究(六项研究)。此外,研究层面的偏倚至关重要.这篇综述表明,职位和纳米材料的产量与8-OHdG的存在之间存在显着关系。此外,可以在尿液中测量8-OHdG的水平,血,和通过仪器程序吸入样品。氧化损伤是接触纳米材料的工人面临的重要威胁。血液和EBC8-OHdG水平可以作为金属纳米材料的生物标志物,但尿中8-OHdG需要谨慎服用。所以,建议评估不能仅基于一种生物标志物.
In nuclear and mitochondrial DNA, 8-hydroxy-2\'-deoxyguanosine (8-OHdG) is one of the predominant forms of reactive oxygen species (ROSs) lesions, which commonly used as a biomarker for oxidative stress. Studies showed that the different nanomaterials can induce toxicity by ROSs in human body. So, this study is going to
review the studies about oxidative DNA damage caused by occupational exposure to nanomaterials, using 8-OHdG biomarker.Systematic
review was managed based on Cochrane systematic
review guideline. Literature search was conducted in scientific databases with the main terms of \"biomarkers,\" \"biological markers,\" combined with \"occupational exposure\" and \"nanomaterials.\" All papers in the field of occupational exposure to nanomaterials until 2020 December were included. To evaluate the quality and bias of studies, GRADE method (Grading of Recommendations, Assessment, Development, and Evaluation) was used.Two hundred twenty-six studies were primarily achieved. By considering the inclusion criteria, overall 8 articles were selected. The majority of the studies were classified as the moderate quality studies (six studies). Also, the study-level bias was critical. This
review shows that there is a significant relationship between job title and amount of produced nanomaterials and the existence of 8-OHdG. Also, the levels of 8-OHdG can be measured in urine, blood, and inhalation samples by instrumental procedures.Oxidative damages are an important threat for workers exposed to nanomaterial. Blood and EBC 8-OHdG level can be introduced as a biomarker for metal nanomaterials, but urinary 8-OHdG needs to be taken with caution. So, it is recommended that evaluation not be solely based on one biomarker.