Template properties of DNA alkylated with N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea.

用 N - 甲基 - N - 亚硝基脲和 N - 乙基 - N - 亚硝基脲烷基化的 DNA 的模板性质。

• 影响指数: 5.168
• 发表时间: Sep 1983 1
• 来源期刊: Chem Biol Interact
• DOI: 10.1016/0009-2797(83)90027-3
• PMID: 6354490
• 文章类型: Comparative Study
• 中科院分区: Q2
• 方向: 生物
• 作者列表: Marushige K,Marushige Y

Mesh: Alkylation; Animals; Cattle; DNA / genetics; DNA-Directed RNA Polymerases / metabolism; Escherichia coli / enzymology; Ethylnitrosourea / metabolism toxicity; Kinetics; Methylnitrosourea / metabolism toxicity; Nitrosourea Compounds / toxicity; Structure-Activity Relationship; Templates, Genetic; Thymus Gland / metabolism; Transcription, Genetic / drug effects

Abstract

Alkylation of DNA with N-methyl-N-nitrosourea (MeNU) and N-ethyl-N-nitrosourea (EtNU) reduces its ability to support RNA synthesis catalyzed by exogenously added RNA polymerase. It is likely that 7-alkylguanine and alkyl phosphotriester in DNA are mainly responsible for the inhibition of RNA synthesis. The inhibitory effect of alkyl groups varies depending upon divalent metal ions and the type of RNA polymerase used as well as upon the presence of chromosomal proteins on DNA templates. Analyses of RNA products indicate that inhibition occurs primarily at the initiation step.