PDF(Pubmed)
Abstract：
The beekeeping industry plays a crucial role in local economies, contributing significantly to their growth. However, bee colonies often face the threat of American foulbrood (AFB), a dangerous disease caused by the Gram-positive bacterium Paenibacillus larvae (P. l.). While the antibiotic Tylosin has been suggested as a treatment, its bacterial resistance necessitates the search for more effective alternatives. This investigation focused on evaluating the potential of bee venom (BV) and silver nanoparticles (Ag NPs) as antibacterial agents against AFB. In vitro treatments were conducted using isolated AFB bacterial samples, with various concentrations of BV and Ag NPs (average size: 25nm) applied individually and in combination. The treatments were administered under both light and dark conditions. The viability of the treatments was assessed by monitoring the lifespans of treated bees and evaluating the treatment\'s efficiency within bee populations. Promising results were obtained with the use of Ag NPs, which effectively inhibited the progression of AFB. Moreover, the combination of BV and Ag NPs, known as bee venom/silver nanocomposites (BV/Ag NCs), significantly extended the natural lifespan of bees from 27 to 40 days. Notably, oral administration of BV in varying concentrations (1.53, 3.12, and 6.25 mg/mL) through sugary syrup doubled the bees\' lifespan compared to the control group. The study established a significant correlation between the concentration of each treatment and the extent of bacterial inhibition. BV/Ag NCs demonstrated 1.4 times greater bactericidal efficiency under photo-stimulation with visible light compared to darkness, suggesting that light exposure enhances the effectiveness of BV/Ag NCs. The combination of BV and Ag NPs demonstrated enhanced antibacterial efficacy and prolonged honeybee lifespan. These results offer insights that can contribute to the development of safer and more efficient antibacterial agents for maintaining honeybee health.
摘要：
养蜂业在当地经济中起着至关重要的作用，对他们的成长做出了很大的贡献。然而,蜂群经常面临美国蜂群(AFB)的威胁，由革兰氏阳性细菌Paenibacillus幼虫引起的危险疾病（P.l.).虽然抗生素泰乐菌素被建议作为一种治疗方法，它的细菌抗性需要寻找更有效的替代品。这项研究的重点是评估蜂毒（BV）和银纳米颗粒（AgNPs）作为抗AFB的抗菌剂的潜力。使用分离的AFB细菌样品进行体外处理，各种浓度的BV和AgNP（平均尺寸：25nm）单独和组合应用。在光照和黑暗条件下施用治疗。通过监测处理蜜蜂的寿命并评估蜜蜂种群内的处理效率来评估处理的可行性。使用AgNP获得了有希望的结果，有效抑制AFB的进展。此外，BV和AgNPs的组合，被称为蜂毒/银纳米复合材料(BV/AgNCs)，将蜜蜂的自然寿命从27天延长到40天。值得注意的是,与对照组相比,通过含糖糖浆口服不同浓度的BV(1.53,3.12和6.25mg/mL)使蜜蜂的寿命延长了一倍.该研究建立了每种处理的浓度与细菌抑制程度之间的显着相关性。与黑暗相比，BV/AgNCs在可见光光刺激下的杀菌效率高1.4倍，表明光照增强了BV/AgNCs的有效性。BV和AgNP的组合显示出增强的抗菌功效和延长的蜜蜂寿命。这些结果提供了见解，可以有助于开发更安全，更有效的抗菌剂来维持蜜蜂的健康。
