Abstract：
Chilling stress is a major environmental factor that significantly reduces crop production. To adapt to chilling stress, plants activate a series of cellular responses and accumulate an array of metabolites, particularly proline. Here, we report that the transcription factor SlWRKY51 increases proline contents in tomato (Solanum lycopersicum) under chilling stress. SlWRKY51 expression is induced under chilling stress. Knockdown or knockout of SlWRKY51 led to chilling-sensitive phenotypes, with lower photosynthetic capacity and more reactive oxygen species (ROS) accumulation than the wild type (WT). The proline contents were significantly reduced in SlWRKY51 knockdown and knockout lines under chilling stress, perhaps explaining the phenotypes of these lines. D-1-pyrroline-5-carboxylate synthetase (P5CS), which catalyses the rate-limiting step of proline biosynthesis, is encoded by two closely related P5CS genes (P5CS1 and P5CS2). We demonstrate that SlWRKY51 directly activates the expression of P5CS1 under chilling stress. In addition, the VQ (a class of plant-specific proteins containing the conserved motif FxxhVQxhTG) family member SlVQ10 physically interacts with SlWRKY51 to enhance its activation of P5CS1. Our study reveals that the chilling-induced transcription factor SlWRKY51 enhances chilling tolerance in tomato by promoting proline accumulation.
摘要：
低温胁迫是显著降低作物产量的主要环境因素。为了适应寒冷的压力，植物激活一系列细胞反应并积累一系列代谢物，尤其是脯氨酸。这里,我们报告说，转录因子SlWRKY51在低温胁迫下增加了番茄（Solanumlycopersicum）中的脯氨酸含量。在低温胁迫下诱导SlWRKY51表达。敲除或敲除SlWRKY51导致寒冷敏感表型，与野生型（WT）相比，具有较低的光合能力和更多的活性氧（ROS）积累。在低温胁迫下，SlWRKY51敲除和敲除系的脯氨酸含量显着降低。也许可以解释这些品系的表型。D-1-吡咯啉-5-羧酸合成酶(P5CS),它催化脯氨酸生物合成的限速步骤，由两个密切相关的P5CS基因(P5CS1和P5CS2)编码。我们证明SlWRKY51在低温胁迫下直接激活P5CS1的表达。此外,VQ（一类含有保守基序FxxhVQxhTG的植物特异性蛋白质）家族成员SlVQ10与SlWRKY51物理相互作用，以增强其对P5CS1的激活。我们的研究表明，低温诱导的转录因子SlWRKY51通过促进脯氨酸积累来增强番茄的耐冷性。
