Abstract：
Bacteria can propel themselves by rotating a flagellum or a flagellar bundle. To image this thin structure in motile bacteria, the flagella can be vitally stained with fluorophores. This chapter describes a flagellar staining protocol with the additional possibility of visualizing the cell body. It offers the opportunity to track conformational changes of flagella and simultaneously track the positions of the cell bodies. The additional use of a filter increases the number of motile cells and improves the signal-to-noise ratio of images. The flagellar staining requires a prior introduction of a surface-exposed cysteine, which is not covered in this chapter.
摘要：
细菌可以通过旋转鞭毛或鞭毛束来推动自己。为了在活动细菌中成像这种薄结构，鞭毛可以被荧光团染色。本章描述了鞭毛染色方案，具有可视化细胞体的额外可能性。它提供了跟踪鞭毛构象变化并同时跟踪细胞体位置的机会。额外使用滤波器增加了活动细胞的数量并提高了图像的信噪比。鞭毛染色需要事先引入表面暴露的半胱氨酸，本章未涵盖的内容。
