PDF(Pubmed)
Abstract：
Salvia miltiorrhiza, known as Danshen, is a traditional Chinese medicinal plant with significant cardiovascular benefits, attributed to its secondary metabolites, particularly tanshinones. Despite their medicinal value, tanshinones occur in low natural abundance, necessitating research to increase their content. This study explores the role of the ARF transcription factor (SmARF1) in tanshinone accumulation in Danshen. Overexpressing SmARF1 in hairy roots significantly increased tanshinone levels. EMSA and Dual-LUC assays revealed that SmMYB36, a transcription factor interacting with SmMAPK3, binds to and regulates the SmARF1 promoter. SmMYB36 alone inhibited the expression of SmARF1 gene, while its interaction with SmMAPK3 enhanced SmARF1 promoter activity. This MAPK3-MYB36-ARF1 module elucidates a complex regulatory mechanism for tanshinone biosynthesis, offering insights for targeted enhancement of tanshinone content through advanced biotechnological approaches.
摘要：
丹参,被称为丹参，是一种具有显著心血管益处的传统中药植物，归因于其次级代谢产物，尤其是丹参酮。尽管它们具有药用价值,丹参酮的自然丰度低，需要研究以增加其内容。本研究探讨了ARF转录因子（SmARF1）在丹参中丹参酮积累中的作用。在毛状根中过度表达SmARF1可显着增加丹参酮水平。EMSA和Dual-LUC分析显示，SmMYB36是一种与SmMAPK3相互作用的转录因子，可结合并调节SmARF1启动子。单独使用SmMYB36抑制SmARF1基因的表达,而其与SmMAPK3的相互作用增强了SmARF1启动子的活性。这个MAPK3-MYB36-ARF1模块阐明了丹参酮生物合成的复杂调控机制，通过先进的生物技术方法提供有针对性地增强丹参酮含量的见解。
