PDF(Pubmed)
Abstract：
Phlomoides umbrosa Turczaninow (PU), a traditional Korean medicinal herb, exhibits osteogenic and anti-inflammatory effects. This research explored the effect of PU extracts on hyperimmune responses within the respiratory tract using lipopolysaccharide-stimulated RAW 264.7 cells and an ovalbumin-induced hyper-responsiveness model. The inflammatory cytokines, protein expression linked to airway inflammation, antioxidant enzyme activity, histopathological observation, and expectorant activity were measured. The results revealed that PU treatment led to a concentration-dependent reduction in Th2 cytokines and the expression of nuclear factor (NF)-κB, phosphatase-tensin homolog, mitogen-activated protein kinase (MAPK), and inducible nitric oxide synthase (iNOS). Simultaneously, antioxidant enzyme activity increased. Furthermore, PU exhibited substantial enhancements in lung tissue condition and expectorant activity relative to the allergic rhinitis-induced group. These findings indicate the potential of PU to mitigate airway inflammation and excessive mucus production by suppressing NF-κB, MAPK, and iNOS pathways. Consequently, PU emerges as a promising anti-inflammatory agent for respiratory tract applications.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s10068-024-01521-3.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s10068-024-01521-3.
摘要：
：一种传统的韩国药草，显示成骨和抗炎作用。这项研究使用脂多糖刺激的RAW264.7细胞和卵清蛋白诱导的高反应性模型探索了PU提取物对呼吸道内超免疫反应的影响。炎症细胞因子，与气道炎症相关的蛋白质表达，抗氧化酶活性，组织病理学观察,并测定祛痰活性。结果表明，PU处理导致Th2细胞因子的浓度依赖性降低和核因子（NF）-κB的表达，磷酸酶-张力蛋白同源物，丝裂原活化蛋白激酶(MAPK),和诱导型一氧化氮合酶（iNOS）。同时,抗氧化酶活性增加。此外，与过敏性鼻炎诱导组相比，PU在肺组织状况和祛痰活性方面表现出实质性增强。这些发现表明PU通过抑制NF-κB减轻气道炎症和过度粘液产生的潜力，MAPK,和iNOS通路。因此,PU作为用于呼吸道应用的有前途的抗炎剂出现。
■在线版本包含补充材料，可在10.1007/s10068-024-01521-3获得。
■在线版本包含补充材料，可在10.1007/s10068-024-01521-3获得。
