PDF(Pubmed)
Abstract：
UNASSIGNED: Breast cancer ranks as one of the most prevalent malignancies among women globally, with increasing incidence rates. Physical activity, particularly exercise, has emerged as a potentially significant modifier of cancer prognosis, influencing tumor biology and patient outcomes.
UNASSIGNED: Using a murine breast cancer model, we established a control and an exercise group, where the latter was subjected to 21 days of voluntary running. RNA Sequencing, bioinformatics analysis, pan-cancer analysis, and cell experiments were performed to validate the underlying mechanisms.
UNASSIGNED: We observed that exercise significantly reduced tumor size and weight, without notable changes in body weight, suggesting that physical activity can modulate tumor dynamics. mRNA sequencing post-exercise revealed substantial downregulation of CD300E in the exercise group, accompanied by alterations in critical pathways such as MicroRNAs in cancers and the Calcium signaling pathway. Expanding our analysis to a broader cancer spectrum, CD300E demonstrated significant expression variability across multiple cancer types, with pronounced upregulation in myeloma, ovarian, lung, and colorectal cancers. This upregulation was correlated with poorer prognostic outcomes, emphasizing CD300E\'s potential role as a prognostic marker and therapeutic target. Moreover, CD300E expression was associated with cancer cell proliferation and apoptosis.
UNASSIGNED: The study highlights the dual role of exercise in modulating gene expression relevant to tumor growth and the potential of CD300E as a target in cancer therapeutics. Further research is encouraged to explore the mechanisms by which exercise and CD300E influence cancer progression and to develop targeted strategies that could enhance patient outcomes in clinical settings.
UNASSIGNED: Using a murine breast cancer model, we established a control and an exercise group, where the latter was subjected to 21 days of voluntary running. RNA Sequencing, bioinformatics analysis, pan-cancer analysis, and cell experiments were performed to validate the underlying mechanisms.
UNASSIGNED: We observed that exercise significantly reduced tumor size and weight, without notable changes in body weight, suggesting that physical activity can modulate tumor dynamics. mRNA sequencing post-exercise revealed substantial downregulation of CD300E in the exercise group, accompanied by alterations in critical pathways such as MicroRNAs in cancers and the Calcium signaling pathway. Expanding our analysis to a broader cancer spectrum, CD300E demonstrated significant expression variability across multiple cancer types, with pronounced upregulation in myeloma, ovarian, lung, and colorectal cancers. This upregulation was correlated with poorer prognostic outcomes, emphasizing CD300E\'s potential role as a prognostic marker and therapeutic target. Moreover, CD300E expression was associated with cancer cell proliferation and apoptosis.
UNASSIGNED: The study highlights the dual role of exercise in modulating gene expression relevant to tumor growth and the potential of CD300E as a target in cancer therapeutics. Further research is encouraged to explore the mechanisms by which exercise and CD300E influence cancer progression and to develop targeted strategies that could enhance patient outcomes in clinical settings.
摘要：
乳腺癌是全球女性中最常见的恶性肿瘤之一，随着发病率的增加。身体活动,特别是锻炼，已经成为癌症预后的潜在重要调节剂，影响肿瘤生物学和患者预后。
■使用鼠乳腺癌模型，我们建立了一个控制和一个锻炼小组，后者接受了21天的自愿跑步。RNA测序,生物信息学分析，泛癌症分析，和细胞实验进行验证的潜在机制。
■我们观察到运动显著降低了肿瘤的大小和重量，体重没有明显变化，表明身体活动可以调节肿瘤动力学。运动后的mRNA测序显示运动组中CD300E的大幅下调，伴随着关键途径的改变，如癌症中的microRNAs和钙信号通路。将我们的分析扩展到更广泛的癌症范围，CD300E在多种癌症类型中表现出显著的表达变异性，骨髓瘤有明显的上调，卵巢,肺，和大肠癌。这种上调与较差的预后结果相关，强调CD300E作为预后标志物和治疗靶点的潜在作用。此外，CD300E表达与癌细胞增殖和凋亡有关。
■该研究强调了运动在调节与肿瘤生长相关的基因表达中的双重作用，以及CD300E作为癌症治疗靶标的潜力。鼓励进一步研究探索运动和CD300E影响癌症进展的机制，并制定可以在临床环境中提高患者预后的有针对性的策略。
■使用鼠乳腺癌模型，我们建立了一个控制和一个锻炼小组，后者接受了21天的自愿跑步。RNA测序,生物信息学分析，泛癌症分析，和细胞实验进行验证的潜在机制。
■我们观察到运动显著降低了肿瘤的大小和重量，体重没有明显变化，表明身体活动可以调节肿瘤动力学。运动后的mRNA测序显示运动组中CD300E的大幅下调，伴随着关键途径的改变，如癌症中的microRNAs和钙信号通路。将我们的分析扩展到更广泛的癌症范围，CD300E在多种癌症类型中表现出显著的表达变异性，骨髓瘤有明显的上调，卵巢,肺，和大肠癌。这种上调与较差的预后结果相关，强调CD300E作为预后标志物和治疗靶点的潜在作用。此外，CD300E表达与癌细胞增殖和凋亡有关。
■该研究强调了运动在调节与肿瘤生长相关的基因表达中的双重作用，以及CD300E作为癌症治疗靶标的潜力。鼓励进一步研究探索运动和CD300E影响癌症进展的机制，并制定可以在临床环境中提高患者预后的有针对性的策略。
