UNASSIGNED: Seven healthy individuals were selected for the study and the blood samples collected from the first five individuals were irradiated to low doses (0-10 cGy) and high doses (50-500 cGy) in a telecobalt unit. All the samples were processed for gamma-H2AX immunofluorescence assay and the dose-response calibration curves for low and high doses were determined. In order to validate the determined dose-response calibration curves, the blood samples obtained from the sixth and seventh subjects were delivered a test dose of 7.5 cGy and 250 cGy. In addition, time and cost required to complete the assay were also reported.
UNASSIGNED: The goodness of fit (R2) values was found to be 0.9829 and 0.9766 for low and high dose-response calibration curves. The time required to perform the gamma-H2AX immunofluorescence assay was found to be 7 hours and 30 minutes and the estimated cost per sample was 5000 rupees (~ 60 USD).
UNASSIGNED: Based on this study we conclude that the individual dose-response calibration curves determined with gamma-H2AX immunofluorescence assay for both low and high dose ranges of gamma radiation can be used for biological dosimetry. Further, the gamma-H2AX immunofluorescence assay can be used as a rapid cost-effective biodosimetric tool for institutions with an existing confocal microscope facility.
■选择了七个健康个体进行研究,并将从前五个个体收集的血液样本以低剂量(0-10cGy)和高剂量(50-500cGy)照射。处理所有样品用于γ-H2AX免疫荧光测定,并确定低剂量和高剂量的剂量反应校准曲线。为了验证确定的剂量反应校准曲线,从第六和第七名受试者获得的血液样本被递送7.5cGy和250cGy的测试剂量。此外,还报告了完成测定所需的时间和成本.
■对于低和高剂量反应校准曲线,拟合优度(R2)值分别为0.9829和0.9766。发现进行γ-H2AX免疫荧光测定所需的时间为7小时30分钟,每个样品的估计成本为5000卢比(约60美元)。
■根据这项研究,我们得出结论,用γ-H2AX免疫荧光测定法测定的低剂量和高剂量范围的γ辐射的个体剂量反应校准曲线可用于生物剂量测定。Further,γ-H2AX免疫荧光测定可用作具有现有共聚焦显微镜设施的机构的快速具有成本效益的生物剂量测定工具。