{Reference Type}: Journal Article
{Title}: Determination of dose-response calibration curves for gamma radiation using gamma-H2AX immunofluorescence based biodosimetry.
{Author}: Jose SR;Timothy PB;Suganthy J;Backianathan S;Amirtham SM;Rani S;Singh R;
{Journal}: Rep Pract Oncol Radiother
{Volume}: 29
{Issue}: 2
{Year}: 2024
暂无{DOI}: 10.5603/rpor.99678
{Abstract}: <B>UNASSIGNED: </B>Gamma-H2AX immunofluorescence assay has gained popularity as a DNA double strand break marker. In this work, we have investigated the potential use of gamma H2AX immunofluorescence assay as a biological dosimeter for estimation of dose in our institution.<BR><B>UNASSIGNED: </B>Seven healthy individuals were selected for the study and the blood samples collected from the first five individuals were irradiated to low doses (0-10 cGy) and high doses (50-500 cGy) in a telecobalt unit. All the samples were processed for gamma-H2AX immunofluorescence assay and the dose-response calibration curves for low and high doses were determined. In order to validate the determined dose-response calibration curves, the blood samples obtained from the sixth and seventh subjects were delivered a test dose of 7.5 cGy and 250 cGy. In addition, time and cost required to complete the assay were also reported.<BR><B>UNASSIGNED: </B>The goodness of fit (R2) values was found to be 0.9829 and 0.9766 for low and high dose-response calibration curves. The time required to perform the gamma-H2AX immunofluorescence assay was found to be 7 hours and 30 minutes and the estimated cost per sample was 5000 rupees (~ 60 USD).<BR><B>UNASSIGNED: </B>Based on this study we conclude that the individual dose-response calibration curves determined with gamma-H2AX immunofluorescence assay for both low and high dose ranges of gamma radiation can be used for biological dosimetry. Further, the gamma-H2AX immunofluorescence assay can be used as a rapid cost-effective biodosimetric tool for institutions with an existing confocal microscope facility.