Abstract：
Graphene oxide (GO) and copper nanoparticles (Cu NPs) were incorporated to modulate and enhance the fluorescence properties of pegylated graphite phase carbon nitride (g-C3N4-PEG). Combined with the specific recognition capability of a molecular imprinted polymer (MIP), a highly sensitive and selective fluorescent molecular imprinted probe for dopamine detection was developed. The fluorescent g-C3N4-PEG was synthesized from melamine and modified with GO and Cu NPs to obtain GO/g-C3N4-PEG@Cu NPs. Subsequently, MIP was prepared on the surface of GO/g-C3N4-PEG@Cu NPs using dopamine as the template molecule. Upon elution of the template molecule, a dopamine-specific GO/g-C3N4-PEG@Cu NPs/MIP fluorescence probe was obtained. The fluorescence intensity of the probe was quenched through the adsorption of different concentrations of dopamine by the MIP, thus establishing a novel method for the detection of dopamine. The linear range of dopamine detection was from 5 × 10-11 to 6 × 10-8 mol L-1, with a detection limit of 2.32 × 10-11 mol L-1. The sensor was utilised for the detection of dopamine in bananas, achieving a spiked recovery rate between 90.3% and 101.3%. These results demonstrate that the fluorescence molecular imprinted sensor developed in this study offers a highly sensitive approach for dopamine detection in bananas.
摘要：
掺入氧化石墨烯（GO）和铜纳米颗粒（CuNPs）以调节和增强聚乙二醇化石墨相氮化碳（g-C3N4-PEG）的荧光特性。结合分子印迹聚合物（MIP）的特定识别能力，开发了一种用于多巴胺检测的高度灵敏和选择性的荧光分子印迹探针。由三聚氰胺合成荧光g-C3N4-PEG，并用GO和CuNPs改性，得到GO/g-C3N4-PEG@CuNPs。随后，使用多巴胺作为模板分子在GO/g-C3N4-PEG@CuNP的表面上制备MIP。在模板分子洗脱时，获得多巴胺特异性GO/g-C3N4-PEG@CuNPs/MIP荧光探针。通过MIP吸附不同浓度的多巴胺,使探针的荧光强度猝灭,从而建立了一种检测多巴胺的新方法。多巴胺检测的线性范围为5×10-11～6×10-8molL-1,检出限为2.32×10-11molL-1。该传感器用于检测香蕉中的多巴胺，达到90.3%至101.3%的峰值回收率。这些结果表明，本研究开发的荧光分子印迹传感器为香蕉中的多巴胺检测提供了高度灵敏的方法。
