PDF(Pubmed)
Abstract：
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) colonization increases the risk of subsequent infection by MRSA strain complex interlinking between hospital and community-acquired MRSA which increases the chance of drug resistance and severity of the disease.
OBJECTIVE: Genomic characterization of Staphylococcus aures strains isolated from patients attending regional referral hospitals in Tanzania.
METHODS: A laboratory-based cross-sectional study using short read-based sequencing technology, (Nextseq550,Illumina, Inc. San diego, California, USA). The samples used were collected from patients attending selected regional referral hospitals in Tanzania under the SeqAfrica project. Sequences were analyzed using tools available in the center for genomic and epidemiology server, and visualization of the phylogenetic tree was performed in ITOL 6.0. SPSS 28.0 was used for statistical analysis.
RESULTS: Among 103 sequences of S. aureus, 48.5% (50/103) carry the mecA gene for MRSA. High proportions of MRSA were observed among participants aged between 18 and 34 years (52.4%), in females (54.3%), and among outpatients (60.5%). The majority of observed MRSA carried plasmids rep5a (92.0%), rep16 (90.0%), rep7c (90.0%), rep15 (82.0%), rep19 (80.0%) and rep10 (72.0%). Among all plasmids observed rep5a, rep16, rep20, and repUS70 carried the blaZ gene, rep10 carried the erm(C) gene and rep7a carried the tet(K) gene. MLST and phylogeny analysis reveal high diversity among MRSA. Six different clones were observed circulating at selected regional hospitals and MRSA with ST8 was dominant.
CONCLUSIONS: The study reveals a significant presence of MRSA in Staphylococcus aureus strains from Tanzanian regional hospitals, with nearly half carrying the mecA gene. MRSA is notably prevalent among young adults, females, and outpatients, showing high genetic diversity and dominance of ST8. Various plasmids carrying resistance genes indicate a complex resistance profile, highlighting the need for targeted interventions to manage MRSA infections in Tanzania.
OBJECTIVE: Genomic characterization of Staphylococcus aures strains isolated from patients attending regional referral hospitals in Tanzania.
METHODS: A laboratory-based cross-sectional study using short read-based sequencing technology, (Nextseq550,Illumina, Inc. San diego, California, USA). The samples used were collected from patients attending selected regional referral hospitals in Tanzania under the SeqAfrica project. Sequences were analyzed using tools available in the center for genomic and epidemiology server, and visualization of the phylogenetic tree was performed in ITOL 6.0. SPSS 28.0 was used for statistical analysis.
RESULTS: Among 103 sequences of S. aureus, 48.5% (50/103) carry the mecA gene for MRSA. High proportions of MRSA were observed among participants aged between 18 and 34 years (52.4%), in females (54.3%), and among outpatients (60.5%). The majority of observed MRSA carried plasmids rep5a (92.0%), rep16 (90.0%), rep7c (90.0%), rep15 (82.0%), rep19 (80.0%) and rep10 (72.0%). Among all plasmids observed rep5a, rep16, rep20, and repUS70 carried the blaZ gene, rep10 carried the erm(C) gene and rep7a carried the tet(K) gene. MLST and phylogeny analysis reveal high diversity among MRSA. Six different clones were observed circulating at selected regional hospitals and MRSA with ST8 was dominant.
CONCLUSIONS: The study reveals a significant presence of MRSA in Staphylococcus aureus strains from Tanzanian regional hospitals, with nearly half carrying the mecA gene. MRSA is notably prevalent among young adults, females, and outpatients, showing high genetic diversity and dominance of ST8. Various plasmids carrying resistance genes indicate a complex resistance profile, highlighting the need for targeted interventions to manage MRSA infections in Tanzania.
摘要：
背景：耐甲氧西林金黄色葡萄球菌（MRSA）的定植增加了医院和社区获得性MRSA之间MRSA菌株复合物的随后感染的风险，这增加了耐药性的机会和疾病的严重程度。
目的：从坦桑尼亚地区转诊医院患者中分离出的金黄色葡萄球菌菌株的基因组特征。
方法：使用基于短读数的测序技术的基于实验室的横断面研究，(Nextseq550,Illumina,Inc.SanDiego,加州,美国）。所使用的样本是从在SeqAfrica项目下在坦桑尼亚选定的地区转诊医院就诊的患者那里收集的。使用基因组和流行病学服务器中心可用的工具分析序列，在ITOL6.0中进行了系统发育树的可视化。采用SPSS28.0进行统计分析。
结果：在金黄色葡萄球菌的103个序列中，48.5%(50/103)携带MRSA的mecA基因。在18至34岁的参与者中观察到高比例的MRSA（52.4％）。女性(54.3%)，门诊病人(60.5%)。大多数观察到的MRSA携带质粒rep5a(92.0%)，rep16（90.0%），rep7c（90.0%），回购15（82.0%），rep19（80.0%）和rep10（72.0%）。在所有观察到的质粒rep5a中，rep16、rep20和repUS70携带blaZ基因,rep10携带erm（C）基因，rep7a携带tet（K）基因。MLST和系统发育分析揭示了MRSA之间的高度多样性。在选定的区域医院中观察到六个不同的克隆在循环，以ST8为主的MRSA。
结论：该研究表明，来自坦桑尼亚地区医院的金黄色葡萄球菌菌株中存在大量MRSA，近一半携带mecA基因。MRSA在年轻人中尤为普遍，女性,和门诊病人，表现出较高的遗传多样性和ST8优势。携带抗性基因的各种质粒表明了复杂的抗性特征，强调需要有针对性的干预措施来管理坦桑尼亚的MRSA感染。
目的：从坦桑尼亚地区转诊医院患者中分离出的金黄色葡萄球菌菌株的基因组特征。
方法：使用基于短读数的测序技术的基于实验室的横断面研究，(Nextseq550,Illumina,Inc.SanDiego,加州,美国）。所使用的样本是从在SeqAfrica项目下在坦桑尼亚选定的地区转诊医院就诊的患者那里收集的。使用基因组和流行病学服务器中心可用的工具分析序列，在ITOL6.0中进行了系统发育树的可视化。采用SPSS28.0进行统计分析。
结果：在金黄色葡萄球菌的103个序列中，48.5%(50/103)携带MRSA的mecA基因。在18至34岁的参与者中观察到高比例的MRSA（52.4％）。女性(54.3%)，门诊病人(60.5%)。大多数观察到的MRSA携带质粒rep5a(92.0%)，rep16（90.0%），rep7c（90.0%），回购15（82.0%），rep19（80.0%）和rep10（72.0%）。在所有观察到的质粒rep5a中，rep16、rep20和repUS70携带blaZ基因,rep10携带erm（C）基因，rep7a携带tet（K）基因。MLST和系统发育分析揭示了MRSA之间的高度多样性。在选定的区域医院中观察到六个不同的克隆在循环，以ST8为主的MRSA。
结论：该研究表明，来自坦桑尼亚地区医院的金黄色葡萄球菌菌株中存在大量MRSA，近一半携带mecA基因。MRSA在年轻人中尤为普遍，女性,和门诊病人，表现出较高的遗传多样性和ST8优势。携带抗性基因的各种质粒表明了复杂的抗性特征，强调需要有针对性的干预措施来管理坦桑尼亚的MRSA感染。
