Abstract：
OBJECTIVE: To study the effects of resveratrol on heroin addiction-related behaviors and to preliminarily explore the possible intervention mechanism of resveratrol in heroin dependence.
METHODS: The effects of resveratrol on heroin withdrawal symptoms were observed by naloxone; The effect of resveratrol on heroin reward memory acquisition was detected by CPP paradigm; The effect of resveratrol on the mental excitability of heroin was tested by open field experiment; The effect of resveratrol on heroin spatial learning and memory was tested by water maze test. Western blot was used to detect Sirtuin 1 (SIRT1) Expression of brain-derived neurotrophic factor (BDNF), glial cell derived neurotrophic factor (GDNF), and postsynaptic density protein (PSD95).
RESULTS: The behavioral results showed that the withdrawal behavior of the resveratrol intervention group was reduced compared with the heroin chronic dependence group (P<0.05), and the shift score of the conditioned place preference test of the resveratrol intervention group was reduced compared with the heroin chronic dependence group (P<0.05) The spatial learning and memory ability of the water maze in the resveratrol intervention group was improved compared with the heroin chronic dependence group (P<0.05), and the mental excitability of the resveratrol intervention group was lower than that of the heroin chronic dependence group (P<0.05), but higher than that of the saline group (P<0.05); SIRT1 The expression levels of BDNF, GDNF and PSD95 protein were significantly increased (P<0.05).
CONCLUSIONS: The behavioral results of this study suggest that resveratrol can be used as a potential drug to treat heroin dependence. At the same time, SIRT1 The expression of BDNF, GDNF, and PSD95 increased; SIRT1, BDNF, GDNF, and PSD95 play an essential role in heroin addiction.
METHODS: The effects of resveratrol on heroin withdrawal symptoms were observed by naloxone; The effect of resveratrol on heroin reward memory acquisition was detected by CPP paradigm; The effect of resveratrol on the mental excitability of heroin was tested by open field experiment; The effect of resveratrol on heroin spatial learning and memory was tested by water maze test. Western blot was used to detect Sirtuin 1 (SIRT1) Expression of brain-derived neurotrophic factor (BDNF), glial cell derived neurotrophic factor (GDNF), and postsynaptic density protein (PSD95).
RESULTS: The behavioral results showed that the withdrawal behavior of the resveratrol intervention group was reduced compared with the heroin chronic dependence group (P<0.05), and the shift score of the conditioned place preference test of the resveratrol intervention group was reduced compared with the heroin chronic dependence group (P<0.05) The spatial learning and memory ability of the water maze in the resveratrol intervention group was improved compared with the heroin chronic dependence group (P<0.05), and the mental excitability of the resveratrol intervention group was lower than that of the heroin chronic dependence group (P<0.05), but higher than that of the saline group (P<0.05); SIRT1 The expression levels of BDNF, GDNF and PSD95 protein were significantly increased (P<0.05).
CONCLUSIONS: The behavioral results of this study suggest that resveratrol can be used as a potential drug to treat heroin dependence. At the same time, SIRT1 The expression of BDNF, GDNF, and PSD95 increased; SIRT1, BDNF, GDNF, and PSD95 play an essential role in heroin addiction.
摘要：
目的：研究白藜芦醇对海洛因成瘾相关行为的影响，初步探讨白藜芦醇干预海洛因依赖的可能机制。
方法:采用纳洛酮观察白藜芦醇对海洛因戒断症状的影响;采用CPP范式检测白藜芦醇对海洛因奖赏记忆获得的影响;采用开场实验检测白藜芦醇对海洛因精神兴奋性的影响;采用水迷宫实验检测白藜芦醇对海洛因空间学习记忆的影响。Westernblot检测Sirtuin1(SIRT1)在脑源性神经营养因子(BDNF)中的表达,胶质细胞源性神经营养因子(GDNF),和突触后密度蛋白(PSD95)。
结果:行为学结果显示,白藜芦醇干预组较海洛因慢性依赖组戒断行为减少(P<0.05),白藜芦醇干预组水迷宫空间学习记忆能力较海洛因慢性依赖组提高(P<0.05),白藜芦醇干预组的精神兴奋性低于海洛因慢性依赖组(P<0.05),但高于生理盐水组(P<0.05);SIRT1中BDNF的表达水平,GDNF和PSD95蛋白明显升高(P<0.05)。
结论：这项研究的行为结果表明，白藜芦醇可以用作治疗海洛因依赖的潜在药物。同时,SIRT1BDNF的表达,GDNF,和PSD95增加；SIRT1，BDNF，GDNF,PSD95在海洛因成瘾中起着至关重要的作用。
方法:采用纳洛酮观察白藜芦醇对海洛因戒断症状的影响;采用CPP范式检测白藜芦醇对海洛因奖赏记忆获得的影响;采用开场实验检测白藜芦醇对海洛因精神兴奋性的影响;采用水迷宫实验检测白藜芦醇对海洛因空间学习记忆的影响。Westernblot检测Sirtuin1(SIRT1)在脑源性神经营养因子(BDNF)中的表达,胶质细胞源性神经营养因子(GDNF),和突触后密度蛋白(PSD95)。
结果:行为学结果显示,白藜芦醇干预组较海洛因慢性依赖组戒断行为减少(P<0.05),白藜芦醇干预组水迷宫空间学习记忆能力较海洛因慢性依赖组提高(P<0.05),白藜芦醇干预组的精神兴奋性低于海洛因慢性依赖组(P<0.05),但高于生理盐水组(P<0.05);SIRT1中BDNF的表达水平,GDNF和PSD95蛋白明显升高(P<0.05)。
结论：这项研究的行为结果表明，白藜芦醇可以用作治疗海洛因依赖的潜在药物。同时,SIRT1BDNF的表达,GDNF,和PSD95增加；SIRT1，BDNF，GDNF,PSD95在海洛因成瘾中起着至关重要的作用。
