Abstract：
We describe a nitrogen-specific detector (NSD) for aqueous mobile phase-based high performance liquid chromatography (HPLC). It is based by means of total hydrophilic organic nitrogen detection. Separated analytes are photooxidized online and converted to nitrate, followed by an ultravilet absorbance detector. It features response dependant on the product of nitrogen number in the molecule and its molar concentration, no matter what is ultravilet-absorbing or not. The HPLC equipped with NSD can quantify nitrogen-containing analytes via a sole standard of potassium nitrate for calibration. This results in identical calibration curve for all nitrogen-containing analytes, obviating individual calibration. The limit of detection of NSD is 4.3 μM N/L, and its linear range is up to 4 mM N/L.
摘要：
我们描述了一种用于基于水性流动相的高效液相色谱（HPLC）的氮气特异性检测器（NSD）。它基于总亲水性有机氮检测。分离的分析物在线光氧化并转化为硝酸盐，然后是ultravilet吸光度检测器。它的特性取决于分子中氮数的乘积及其摩尔浓度，不管什么是ultravilet吸收与否。配备有NSD的HPLC可以通过唯一的硝酸钾标准来定量含氮分析物用于校准。这导致所有含氮分析物的校准曲线相同，避免单独校准。NSD的检测限为4.3μMN/L，线性范围高达4mMN/L
