{Reference Type}: Journal Article
{Title}: A nitrogen-specific detector for high performance liquid chromatography.
{Author}: Peng Y;Sun X;Zhang F;Huang W;Yang B;
{Journal}: Talanta
{Volume}: 280
{Issue}: 0
{Year}: 2024 Aug 10
{Factor}: 6.556
{DOI}: 10.1016/j.talanta.2024.126697
{Abstract}: We describe a nitrogen-specific detector (NSD) for aqueous mobile phase-based high performance liquid chromatography (HPLC). It is based by means of total hydrophilic organic nitrogen detection. Separated analytes are photooxidized online and converted to nitrate, followed by an ultravilet absorbance detector. It features response dependant on the product of nitrogen number in the molecule and its molar concentration, no matter what is ultravilet-absorbing or not. The HPLC equipped with NSD can quantify nitrogen-containing analytes via a sole standard of potassium nitrate for calibration. This results in identical calibration curve for all nitrogen-containing analytes, obviating individual calibration. The limit of detection of NSD is 4.3 μM N/L, and its linear range is up to 4 mM N/L.