Abstract：
Extracellular vesicles (EVs) are naturally occurring lipid-bound nanoparticles produced by all cell types. Growing work demonstrates the ability of EVs to facilitate long-distance and cross-kingdom communication. Their innate barrier crossing and cell targeting properties make them a uniquely useful starting ground for novel drug delivery platforms. To better understand the endogenous activity and therapeutic potential of EVs, recent work has measured particle circulation and distribution in vivo using several approaches. Here, we describe molecular-based methods for quantifying bacterial EV distribution in collected tissue samples for biodistribution studies. These methods are important for understanding cell-cell communication facilitated by bacterial EVs and for identifying opportunities for using bacterial EVs as a therapeutic platform.
摘要：
细胞外囊泡(EV)是由所有细胞类型产生的天然存在的脂质结合的纳米颗粒。不断增长的工作证明了电动汽车促进长途和跨国通信的能力。它们的先天屏障交叉和细胞靶向特性使它们成为新型药物递送平台的独特有用起点。为了更好地了解电动汽车的内源性活性和治疗潜力，最近的工作已经测量颗粒循环和分布在体内使用几种方法。这里,我们描述了基于分子的方法，用于定量收集的组织样品中的细菌EV分布，以进行生物分布研究。这些方法对于理解由细菌EV促进的细胞-细胞通信以及确定使用细菌EV作为治疗平台的机会是重要的。
