Abstract：
For the genome-wide mapping of histone modifications, chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing remains the benchmark method. While crosslinked ChIP can be used for all kinds of targets, native ChIP is predominantly used for strong and direct DNA interactors like histones and their modifications. Here we describe a native ChIP protocol that can be used for cells and tissue material.
摘要：
对于组蛋白修饰的全基因组图谱，染色质免疫沉淀（ChIP）随后进行高通量测序仍然是基准方法。虽然交联的ChIP可以用于各种目标，天然ChIP主要用于强且直接的DNA相互作用物，例如组蛋白及其修饰。在这里，我们描述了一种可用于细胞和组织材料的原生ChIP协议。
