Abstract：
Tea, a globally popular beverage, contains various beneficial secondary metabolites. Tea plants (Camellia sinensis) exhibit diverse genetic traits across cultivars, impacting yield, adaptability, morphology, and secondary metabolite composition. Many tea cultivars have been the subject of much research interest, which have led to the accumulation of publicly available RNA-seq data. As such, it has become possible to systematically summarize the characteristics of different cultivars at the transcriptomic level, identify functional genes, and infer gene functions through co-expression analysis. Here, the transcriptomes of 9 tea cultivars were assembled, and comparative analysis was conducted on the coding sequences of 13 cultivars. To give access to this data, we present TeaNekT (https://teanekt.sbs.ntu.edu.sg/), a web resource that facilitates the prediction of gene functions of various tea cultivars. We used TeaNekT to perform a cross-cultivar comparison of co-expressed gene clusters and tissue-specific gene expression. We observed that \'Anji Baicha\' possesses the highest number of cultivar-specific genes and the second-highest number of expanded genes. These genes in \'Anji Baicha\' tend to be enriched in functions associated with cold stress response, chloroplast thylakoid structure, and nitrogen metabolism. Notably, we identified three significantly expanded homologous genes in \'Anji Baicha\' encoding the ICE1, SIZ1, and MAPKK2, which are closely associated with the cold sensitivity of \'Anji Baicha\'. Additionally, one significantly expanded homologous gene in \'Anji Baicha\' encoding regulatory factor RIQ may play a crucial role in the abnormal chloroplast structure and absence of thylakoid membranes in \'Anji Baicha\'.
摘要：
茶，一种全球流行的饮料，含有各种有益的次级代谢产物。茶树（茶树）在不同品种之间表现出不同的遗传特性，影响产量，适应性，形态学,和次级代谢产物组成。许多茶树品种一直是许多研究兴趣的主题，这导致了公开可用的RNA-seq数据的积累。因此，在转录组水平上系统地总结不同品种的特征已成为可能，识别功能基因，并通过共表达分析推断基因功能。这里,组装了9个茶树品种的转录组，并对13个品种的编码序列进行了比较分析。为了访问这些数据，我们介绍TeaNekT(https://teanekt.sbs.ntu.edu.sg/),一种网络资源，有助于预测各种茶树品种的基因功能。我们使用TeaNekT进行共表达基因簇和组织特异性基因表达的交叉品种比较。我们观察到\'AnjiBaicha\'拥有最高数量的品种特异性基因和第二高数量的扩展基因。这些基因在\'安吉Baicha\'倾向于丰富与冷应激反应相关的功能，叶绿体类囊体结构，和氮代谢。值得注意的是,我们在编码ICE1，SIZ1和MAPKK2的\''安吉白茶\'中鉴定了三个显着扩展的同源基因，这些基因与\'安吉白茶\'的冷敏感性密切相关。此外,编码调节因子RIQ的“安吉Baicha”中一个显着扩展的同源基因可能在“安吉Baicha”中的叶绿体结构异常和类囊体膜缺失中起关键作用。
