PDF(Pubmed)
Abstract：
BACKGROUND: Sepsis is associated with a highest mortality rate in the ICU. Present study tests the efficacy of combined therapy with vitamin C, hydrocortisone and thiamine (combined therapy) in the ovine model of sepsis induced by Pseudomonas aeruginosa. In this study, sepsis was induced in sheep by instillation of Pseudomonas aeruginosa (1 × 1011 CFU) into the lungs via bronchoscope, under anesthesia. Nine hours after injury, intravenous infusion of vitamin C (0.75 g every 6 h), hydrocortisone (25 mg every 6 h), and thiamine (100 mg every 12 h) or saline was given to the treatment and control groups. Cardiopulmonary variables were recorded.
RESULTS: The survival rate was 16.7% in control and 33.3% in treatment groups. In the control group, mean arterial pressure dropped from 93.6 ± 8.6 to 75.5 ± 9.7 mmHg by 9 h, which was not affected by the combined therapy. Pulmonary dysfunction was not attenuated by the combined therapy either. The combined therapy had no effect on increased extravascular lung water content and fluid effusion into thoracic cavity. The bacterial number in the bronchoalveolar lavage fluid was significantly increased in the treatment group than the control group. The blood bacterial number remained comparable between groups.
CONCLUSIONS: Combined vitamin C, hydrocortisone, and thiamine did not attenuate severity of ovine sepsis.
RESULTS: The survival rate was 16.7% in control and 33.3% in treatment groups. In the control group, mean arterial pressure dropped from 93.6 ± 8.6 to 75.5 ± 9.7 mmHg by 9 h, which was not affected by the combined therapy. Pulmonary dysfunction was not attenuated by the combined therapy either. The combined therapy had no effect on increased extravascular lung water content and fluid effusion into thoracic cavity. The bacterial number in the bronchoalveolar lavage fluid was significantly increased in the treatment group than the control group. The blood bacterial number remained comparable between groups.
CONCLUSIONS: Combined vitamin C, hydrocortisone, and thiamine did not attenuate severity of ovine sepsis.
摘要：
背景：脓毒症与ICU死亡率最高相关。本研究测试了维生素C联合治疗的疗效，氢化可的松和硫胺素（联合疗法）在铜绿假单胞菌诱导的脓毒症绵羊模型中的作用。在这项研究中,通过支气管镜将铜绿假单胞菌（1×1011CFU）滴入肺部，在绵羊中诱发败血症，在麻醉下。受伤后九小时，静脉输注维生素C（每6小时0.75g），氢化可的松（25毫克每6小时），治疗组和对照组给予硫胺素（每12h100mg）或生理盐水。记录心肺变量。
结果：对照组生存率为16.7%，治疗组为33.3%。在对照组中，平均动脉压9小时从93.6±8.6下降到75.5±9.7mmHg，不受联合治疗的影响。联合治疗也未减轻肺功能障碍。联合治疗对增加血管外肺含水量和胸腔积液没有影响。治疗组支气管肺泡灌洗液中细菌数量明显高于对照组。各组之间的血液细菌数量保持相当。
结论：复合维生素C，氢化可的松,硫胺素并不能减轻绵羊脓毒症的严重程度。
结果：对照组生存率为16.7%，治疗组为33.3%。在对照组中，平均动脉压9小时从93.6±8.6下降到75.5±9.7mmHg，不受联合治疗的影响。联合治疗也未减轻肺功能障碍。联合治疗对增加血管外肺含水量和胸腔积液没有影响。治疗组支气管肺泡灌洗液中细菌数量明显高于对照组。各组之间的血液细菌数量保持相当。
结论：复合维生素C，氢化可的松,硫胺素并不能减轻绵羊脓毒症的严重程度。
