PDF(Pubmed)
Abstract：
BACKGROUND: Numerous insect species undertake long-distance migrations on an enormous scale, with great implications for ecosystems. Given that take-off is the point where it all starts, whether and how the external light and internal circadian rhythm are involved in regulating the take-off behaviour remains largely unknown. Herein, we explore this issue in a migratory pest, Cnaphalocrocis medinalis, via behavioural observations and RNAi experiments.
RESULTS: The results showed that C. medinalis moths took off under conditions where the light intensity gradually weakened to 0.1 lx during the afternoon or evening, and the take-off proportions under full spectrum or blue light were significantly higher than that under red and green light. The ultraviolet-A/blue light-sensitive type 1 cryptochrome gene (Cmedcry1) was significantly higher in take-off moths than that of non-take-off moths. In contrast, the expression of the light-insensitive CRY2 (Cmedcry2) and circadian genes (Cmedtim and Cmedper) showed no significant differences. After silencing Cmedcry1, the take-off proportion significantly decreased. Thus, Cmedcry1 is involved in the decrease in light intensity induced take-off behaviour in C. medinalis.
CONCLUSIONS: This study can help further explain the molecular mechanisms behind insect migration, especially light perception and signal transmission during take-off phases.
RESULTS: The results showed that C. medinalis moths took off under conditions where the light intensity gradually weakened to 0.1 lx during the afternoon or evening, and the take-off proportions under full spectrum or blue light were significantly higher than that under red and green light. The ultraviolet-A/blue light-sensitive type 1 cryptochrome gene (Cmedcry1) was significantly higher in take-off moths than that of non-take-off moths. In contrast, the expression of the light-insensitive CRY2 (Cmedcry2) and circadian genes (Cmedtim and Cmedper) showed no significant differences. After silencing Cmedcry1, the take-off proportion significantly decreased. Thus, Cmedcry1 is involved in the decrease in light intensity induced take-off behaviour in C. medinalis.
CONCLUSIONS: This study can help further explain the molecular mechanisms behind insect migration, especially light perception and signal transmission during take-off phases.
摘要：
背景：许多昆虫物种进行大规模的远距离迁徙，对生态系统有重大影响。鉴于起飞是一切开始的地方，外部光和内部昼夜节律是否以及如何参与调节起飞行为仍然是未知的。在这里，我们在迁徙害虫中探索这个问题，稻长,通过行为观察和RNAi实验。
结果：结果表明，在下午或晚上光照强度逐渐减弱至0.1lx的条件下，大黄蛾起飞，全光谱或蓝光下的起飞比例明显高于红光和绿光下的起飞比例。紫外线A/蓝光敏感的1型隐色素基因（Cmedcry1）在起飞蛾中明显高于非起飞蛾。相比之下,对光不敏感的CRY2（Cmedcry2）和昼夜节律基因（Cmedtim和Cmedper）的表达没有显着差异。沉默Cmedcry1后，起飞比例显著下降。因此，Cmedcry1参与了光强度的降低，引起了黄梭菌的起飞行为。
结论：这项研究可以帮助进一步解释昆虫迁移背后的分子机制，特别是起飞阶段的光感知和信号传输。
结果：结果表明，在下午或晚上光照强度逐渐减弱至0.1lx的条件下，大黄蛾起飞，全光谱或蓝光下的起飞比例明显高于红光和绿光下的起飞比例。紫外线A/蓝光敏感的1型隐色素基因（Cmedcry1）在起飞蛾中明显高于非起飞蛾。相比之下,对光不敏感的CRY2（Cmedcry2）和昼夜节律基因（Cmedtim和Cmedper）的表达没有显着差异。沉默Cmedcry1后，起飞比例显著下降。因此，Cmedcry1参与了光强度的降低，引起了黄梭菌的起飞行为。
结论：这项研究可以帮助进一步解释昆虫迁移背后的分子机制，特别是起飞阶段的光感知和信号传输。
