RESULTS: The results showed that C. medinalis moths took off under conditions where the light intensity gradually weakened to 0.1 lx during the afternoon or evening, and the take-off proportions under full spectrum or blue light were significantly higher than that under red and green light. The ultraviolet-A/blue light-sensitive type 1 cryptochrome gene (Cmedcry1) was significantly higher in take-off moths than that of non-take-off moths. In contrast, the expression of the light-insensitive CRY2 (Cmedcry2) and circadian genes (Cmedtim and Cmedper) showed no significant differences. After silencing Cmedcry1, the take-off proportion significantly decreased. Thus, Cmedcry1 is involved in the decrease in light intensity induced take-off behaviour in C. medinalis.
CONCLUSIONS: This study can help further explain the molecular mechanisms behind insect migration, especially light perception and signal transmission during take-off phases.
结果:结果表明,在下午或晚上光照强度逐渐减弱至0.1lx的条件下,大黄蛾起飞,全光谱或蓝光下的起飞比例明显高于红光和绿光下的起飞比例。紫外线A/蓝光敏感的1型隐色素基因(Cmedcry1)在起飞蛾中明显高于非起飞蛾。相比之下,对光不敏感的CRY2(Cmedcry2)和昼夜节律基因(Cmedtim和Cmedper)的表达没有显着差异。沉默Cmedcry1后,起飞比例显著下降。因此,Cmedcry1参与了光强度的降低,引起了黄梭菌的起飞行为。
结论:这项研究可以帮助进一步解释昆虫迁移背后的分子机制,特别是起飞阶段的光感知和信号传输。