RESULTS: In the hybrid, there were 8,126 and 1,610 different peaks for H3K4me3 and H3K27me3 modifications when compared to its parents, respectively, with the majority of them originating from the parental lines. The different modifications between the hybrid and its parents were more frequently observed as higher levels in the hybrid than in the parents. In ZY19, there were 476 and 84 allele-specific genes with H3K4me3 and H3K27me3 modifications identified, representing 7.9% and 12% of the total analyzed genes, respectively. Only a small portion of genes that showed differences in parental H3K4me3 and H3K27me3 modifications which demonstrated allele-specific histone modifications (ASHM) in the hybrid. The H3K4me3 modification level in the hybrid was significantly lower compared to the parents. In the hybrid, DNA methylation occurs more frequently among histone modification target genes. Additionally, over 62.58% of differentially expressed genes (DEGs) were affected by epigenetic variations. Notably, there was a strong correlation observed between variations in H3K4me3 modifications and gene expression levels in the hybrid and its parents.
CONCLUSIONS: These findings highlight the substantial impact of histone modifications and DNA methylation on gene expression during hybridization. Epigenetic variations play a crucial role in controlling the differential expression of genes, with potential implications for heterosis.
结果:在混合动力车中,与它的亲本相比,H3K4me3和H3K27me3修饰有8,126和1,610个不同的峰,分别,其中大多数来自父母系。杂种与其亲本之间的不同修饰更频繁地观察到,杂种中的水平高于亲本中的水平。在ZY19中,有476和84个等位基因特异性基因与H3K4me3和H3K27me3修饰鉴定,占总分析基因的7.9%和12%,分别。只有一小部分在亲本H3K4me3和H3K27me3修饰中显示差异的基因在杂种中表现出等位基因特异性组蛋白修饰(ASHM)。杂种中的H3K4me3修饰水平显著低于亲本。在混合动力车中,DNA甲基化在组蛋白修饰靶基因中更频繁地发生。此外,超过62.58%的差异表达基因(DEGs)受到表观遗传变异的影响。值得注意的是,在杂种及其亲本中,H3K4me3修饰的变异与基因表达水平之间存在很强的相关性。
结论:这些发现强调了组蛋白修饰和DNA甲基化对杂交过程中基因表达的实质性影响。表观遗传变异在控制基因的差异表达中起着至关重要的作用,对杂种优势有潜在的影响。