Abstract：
The use of time-lapse live imaging enables us to track the dynamic changes in neurites during their formation. Ex vivo live imaging with acute brain slices provides a more physiological environment than cultured cells. To accomplish this, a certain method of labeling is necessary to visualize and identify neurite morphology. To understand the dynamics of neurite structure at early stages of neurite formation, we describe in this chapter ex vivo live imaging using a confocal microscope at P0 in combination with in utero electroporation (IUE).
摘要：
延时实时成像的使用使我们能够跟踪神经突形成过程中的动态变化。使用急性脑切片的离体活体成像提供了比培养细胞更生理的环境。要做到这一点，一定的标记方法是必要的可视化和识别神经突形态。为了了解神经突形成早期神经突结构的动力学，我们在本章中描述了在P0使用共聚焦显微镜结合子宫内电穿孔（IUE）的离体活体成像。
