Abstract：
Wounds or chronic injuries are associated with high medical costs so, develop healing-oriented drugs is a challenge for modern medicine. The identification of new therapeutic alternatives focuses on the use of natural products. Therefore, the main goal of this study was to evaluate the healing potential and anti-inflammatory mechanism of action of extracts and the main compounds derived from Myrciaria plinioides D. Legrand leaves. The antimicrobial activity of leaf extracts was analyzed. Cell viability, cytotoxicity and genotoxicity of plant extracts and compounds were also assessed. Release of pro- and anti-inflammatory cytokines and TGF-β by ELISA, and protein expression was determined by Western Blot. The cell migration and cell proliferation of ethanol and aqueous leaf extracts and p-coumaric acid, quercetin and caffeic acid compounds were also evaluated. The aqueous extract exhibited antibacterial activity and, after determining the safety concentrations in three assays, we showed that this extract induced p38-α MAPK phosphorylation and the same extract and the p-coumaric acid decreased COX-2 and caspase-3, -8 expression, as well as reduced the TNF-α release and stimulated the IL-10 in RAW 264.7 cells. In L929 cells, the extract and p-coumaric acid induced TGF-β release, besides increasing the process of cell migration and proliferation. These results suggested that the healing properties of Myrciaria plinioides aqueous extract can be associated to the presence of phenolic compounds, especially p-coumaric acid, and/or glycosylated metabolites.
摘要：
创伤或慢性损伤与高昂的医疗费用有关，开发以治疗为导向的药物是现代医学面临的挑战。新的治疗替代品的识别集中在天然产物的使用上。因此,这项研究的主要目的是评估提取物和来自MyrciariaplinioidesD.Legrand叶的主要化合物的愈合潜力和抗炎作用机制。分析了叶提取物的抗菌活性。细胞活力，还评估了植物提取物和化合物的细胞毒性和遗传毒性。通过ELISA检测促炎和抗炎细胞因子和TGF-β的释放，蛋白质表达通过蛋白质印迹测定。乙醇和水叶提取物和对香豆酸的细胞迁移和细胞增殖，槲皮素和咖啡酸化合物也进行了评估。水提取物具有抗菌活性,在确定了三个测定的安全浓度后，我们表明，该提取物诱导p38-αMAPK磷酸化和相同的提取物和对香豆酸降低COX-2和caspase-3，-8表达，以及减少TNF-α释放并刺激RAW264.7细胞中的IL-10。在L929细胞中,提取物和对香豆酸诱导TGF-β释放，除了增加细胞迁移和增殖的过程。这些结果表明，Myrciariaplinioides水提取物的愈合特性可能与酚类化合物的存在有关，尤其是对香豆酸,和/或糖基化代谢物。
