PDF(Pubmed)
Abstract：
Whole genome sequencing (WGS) has become a vital tool in clinical microbiology, playing an important role in outbreak investigations, molecular surveillance, and identification of bacterial species, resistance mechanisms and virulence factors. However, the complexity of WGS data presents challenges in interpretation and reporting, requiring tailored strategies to enhance efficiency and impact. This study explores the diverse needs of key stakeholders in healthcare, including clinical management, laboratory work, public surveillance and epidemiology, infection prevention and control, and academic research, regarding WGS-based reporting of clinically relevant bacterial species. In order to determine preferences regarding WGS reports, human-centered design approach was employed, involving an online survey and a subsequent workshop with stakeholders. The survey gathered responses from 64 participants representing the above mentioned healthcare sectors across geographical regions. Key findings include the identification of barriers related to data accessibility, integration with patient records, and the complexity of interpreting WGS results. As the participants designed their ideal report using nine pre-defined sections of a typical WGS report, differences in needs regarding report structure and content across stakeholders became evident. The workshop discussions further highlighted the need to feature critical findings and quality metrics prominently in reports, as well as the demand for flexible report designs. Commonalities were observed across stakeholder-specific reporting templates, such as the uniform ranking of certain report sections, but preferences regarding the depth of content within these sections varied. Using these findings, we suggest stakeholder-specific structures which should be considered when designing customized reporting templates. In conclusion, this study underscores the importance of tailoring WGS-based reports of clinically relevant bacteria to meet the distinct needs of diverse healthcare stakeholders. The evolving landscape of digital reporting increases the opportunities with respect to WGS reporting and its utility in managing infectious diseases and public health surveillance.
摘要：
全基因组测序（WGS）已成为临床微生物学的重要工具，在疫情调查中发挥重要作用，分子监测,和细菌种类的鉴定，抗性机制和毒力因子。然而,WGS数据的复杂性给解释和报告带来了挑战，需要量身定制的策略来提高效率和影响。这项研究探讨了医疗保健关键利益相关者的多样化需求，包括临床管理，实验室工作,公共监测和流行病学，感染预防和控制，和学术研究，关于基于WGS的临床相关细菌种类报告。为了确定有关WGS报告的偏好，采用以人为本的设计方法，涉及在线调查和随后与利益相关者的研讨会。该调查收集了来自64位参与者的答复，这些参与者代表了上述地理区域的医疗保健部门。主要发现包括识别与数据可访问性相关的障碍，与患者记录整合，以及解释WGS结果的复杂性。当参与者使用典型WGS报告的九个预定义部分设计理想报告时，各利益相关者在报告结构和内容方面的需求差异变得显而易见。研讨会的讨论进一步强调，需要在报告中突出重要的调查结果和质量指标，以及灵活的报告设计的需求。在特定于利益相关者的报告模板中观察到了共性，例如某些报告部分的统一排名，但是关于这些部分内容深度的偏好各不相同。利用这些发现，我们建议在设计定制报告模板时应考虑的特定于利益相关者的结构。总之,这项研究强调了定制基于WGS的临床相关细菌报告的重要性,以满足不同医疗保健利益相关者的不同需求.数字报告的不断发展增加了WGS报告的机会及其在管理传染病和公共卫生监测方面的效用。
