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Abstract：
Plasmonic nanomaterials bearing targeting ligands are of great interest for surface-enhanced Raman scattering (SERS)-based bioimaging applications. However, the practical utility of SERS-based imaging strategies has been hindered by the lack of a straightforward method to synthesize highly sensitive SERS-active nanostructures with high yield and efficiency. In this work, leveraging DNA origami principles, we report the first-in-class design of a SERS-based plasmonically coupled nanoprobe for targeted cancer imaging (SPECTRA). The nanoprobe harnesses a cancer cell targeting DNA aptamer sequence and vibrational tag with stretching frequency in the cell-silent Raman window. Through the integration of aptamer sequence specific for DU145 cells, we show the unique capabilities of SPECTRA for targeted imaging of DU145 cells. Our results demonstrate that the scalability, cost-effectiveness, and reproducibility of this method of fabrication of SERS nanoprobes can serve as a versatile platform for creating nanoprobes with broad applications in the fields of cancer biology and biomedical imaging.
摘要：
带有靶向配体的等离子体纳米材料对于基于表面增强拉曼散射（SERS）的生物成像应用非常感兴趣。然而,基于SERS的成像策略的实用性受到缺乏以高产率和高效率合成高灵敏度SERS活性纳米结构的直接方法的阻碍。在这项工作中,利用DNA折纸原理，我们报道了用于靶向癌症成像(SPECTRA)的基于SERS的等离子体耦合纳米探针的一流设计.纳米探针利用靶向癌细胞的DNA适体序列和振动标签,在细胞沉默的拉曼窗口中具有拉伸频率。通过整合DU145细胞特异性的适体序列，我们展示了SPECTRA对DU145细胞靶向成像的独特功能.我们的结果表明，可扩展性，成本效益，这种制造SERS纳米探针的方法的可重复性和可重复性可以作为创建在癌症生物学和生物医学成像领域具有广泛应用的纳米探针的通用平台。
