PDF(Pubmed)
Abstract：
This study aimed to evaluate the survivability of Lactiplantibacillus plantarum PMO08 in the human gastrointestinal tract and its adaptability in the colon using in vitro models. After exposure to gastric and small intestinal conditions, the majority (92.70 ± 1.14%) of PMO08 was found to be damaged, as determined by confocal microscopy and flow cytometry. During in vitro colonic fermentation, PMO08 not only increased abundance up to 0.47 ± 0.04% compared with the control sample (0.00 ± 0.00%) at 24 h but also facilitated the growth of beneficial or commensal bacteria, thereby increasing the α-diversity indices. Additionally, PMO08 significantly elevated the levels of short-chain fatty acids (SCFAs) and various organic acids. Our results demonstrate that PMO08 possesses moderate viability under gastrointestinal conditions but exhibits superior probiotic activity in the colon.
摘要：
本研究旨在使用体外模型评估植物乳杆菌PMO08在人胃肠道中的生存能力及其在结肠中的适应性。暴露于胃和小肠疾病后，大部分(92.70±1.14%)的PMO08被发现损坏，通过共聚焦显微镜和流式细胞术确定。在体外结肠发酵过程中，PMO08不仅在24小时时与对照样品（0.00±0.00％）相比增加了高达0.47±0.04％的丰度，而且还促进了有益或共生细菌的生长，从而增加了α-多样性指数。此外,PM008显著提高了短链脂肪酸(SCFA)和各种有机酸的水平。我们的结果表明，PM008在胃肠道条件下具有中等活力，但在结肠中表现出优异的益生菌活性。
