PDF(Pubmed)
Abstract：
The anthocyanin accumulation in juvenile tissues can enhance the ornamental value, attract pollinators, and help improve abiotic stress. Although transcriptional regulation studies of anthocyanin have been relatively extensive, there are few reports on the mechanism of anthocyanin accumulation in young tissues. This study reveals that many juvenile citrus tissues (flowers, leaves, and pericarp) undergo transient accumulation of anthocyanins, exhibiting a red coloration. Using weighted gene co-expression network analysis (WGCNA) identified CitWRKY75 as a candidate gene. After detecting the expression levels of CitWRKY75 in various citrus juvenile tissues, the expression trend of CitWRKY75 was highly consistent with the red exhibiting and fading. Overexpression of CitWRKY75 in tobacco significantly increased the anthocyanin content. LUC and yeast one-hybrid assay demonstrated that CitWRKY75 could bind to the promoter of CitRuby1(encoding the key transcription factor promoting anthocyanin accumulation) and promote its expression. Finally, comparing the expression levels of CitWRKY75 and CitRuby1 in the late development stage of blood orange found that CitWRKY75 was not the main regulatory factor for anthocyanin accumulation in the later stage. This study used reverse genetics to identify a transcription factor, CitWRKY75, upstream of CitRuby1, which promotes anthocyanin accumulation in citrus juvenile tissues.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01490-9.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01490-9.
摘要：
花青素在幼年组织中的积累可以提高观赏价值，吸引传粉者,并有助于改善非生物胁迫。虽然花青素的转录调控研究相对广泛,关于花色苷在年轻组织中积累的机制的报道很少。这项研究表明，许多幼年柑橘组织(花，叶子,和果皮）经历花色苷的瞬时积累，表现出红色的颜色。使用加权基因共表达网络分析(WGCNA)将CitWRKY75鉴定为候选基因。检测CitWRKY75在各种柑橘幼鱼组织中的表达水平,CitWRKY75的表达趋势与红色表现和褪色高度一致。在烟草中过表达CitWRKY75显著增加了花青素含量。LUC和酵母单杂交实验表明，CitWRKY75可以与CitRuby1的启动子（编码促进花青素积累的关键转录因子）结合并促进其表达。最后,比较CitWRKY75和CitRuby1在血橙发育后期的表达水平发现，CitWRKY75不是后期花青素积累的主要调节因子。这项研究使用反向遗传学来鉴定转录因子，CitWRKY75，位于CitRuby1上游，可促进柑橘幼鱼组织中花青素的积累。
■在线版本包含补充材料，可在10.1007/s11032-024-01490-9获得。
■在线版本包含补充材料，可在10.1007/s11032-024-01490-9获得。
