PDF(Pubmed)
Abstract：
Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 NBPs with discrete autonomous germline functions. By identifying NBPs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.
摘要：
生育力需要生殖细胞的忠实增殖及其分化成配子。控制这些细胞状态需要基因网络的精确时间和表达。核酸结合蛋白（NBPs）在影响生殖细胞发育的基因表达网络中起关键作用。有，然而,在控制体内生殖细胞发育方面，没有对整个NBP库进行功能分析。这里,我们分析了生殖细胞状态和种系结构,系统地研究了364种系表达的NBPs在秀丽隐杆线虫种系中的功能.使用种系特异性敲除，自动生殖细胞计数，以及对生殖细胞细胞核和质膜组织的高含量分析，我们确定了156个具有离散自主种系函数的NBPs。通过识别控制生殖细胞周期的NBPs,扩散,分化,种系结构和生育力，我们为生殖细胞行为和配子产生的机械解剖创建了一个地图集。
