PDF(Pubmed)
Abstract：
Tryptophan (an essential amino acid) and its clinically important metabolite-kynurenine contribute to several fundamental biological processes and methods that allow their determination in biological samples are in demand. The novelty of the work was a demonstration of the utility of two polymers: 4-vinylpyridine crosslinked with trimethylolpropane trimethacrylate (poly(4VP-co-TRIM)) or 1,4-dimethacryloyloxybenzene (poly(4VP-co-14DMB))-in terms of human serum clean-up for simultaneous LC-MS determination of tryptophan and kynurenine. The goal was to achieve a reduction of the matrix effect, which is responsible for signal suppression, with minimal capture of analytes. The adsorption properties of the polymeric beads were studied by evaluating the adsorption kinetics and isotherms in model matrices. Therefore, the adsorption capacities of both molecules were not efficient, the tested 4-vinylpyridine-based copolymers have shown great promise (especially poly(4VP-co-TRIM)) as sorbents for serum clean-up. In the model human serum matrix, poly(4VP-co-TRIM) provided good recoveries of tryptophan and kynurenine (76% and 87%, respectively) and allowed for the reduction of the matrix effect. Performances of both copolymers were compared to those of commercially available sorbents (octadecylsilane, activated charcoal, and primary secondary amine).
摘要：
色氨酸（一种必需氨基酸）及其临床上重要的代谢物犬尿氨酸有助于几种基本的生物过程，并且需要在生物样品中测定它们的方法。这项工作的新颖性证明了两种聚合物的实用性：与三羟甲基丙烷三甲基丙烯酸酯交联的4-乙烯基吡啶（聚（4VP-co-TRIM））或1,4-二甲基丙烯酰氧基苯（聚（4VP-co-14DMB））-在人血清净化中同时LC-MS测定色氨酸和犬尿氨酸。目标是减少基质效应，负责信号抑制，对分析物的捕获最少。通过评估模型基质中的吸附动力学和等温线研究了聚合物珠的吸附性能。因此,两种分子的吸附能力都没有效率，所测试的基于4-乙烯基吡啶的共聚物已经显示出作为用于血清净化的吸附剂的巨大前景(尤其是聚(4VP-co-TRIM))。在人血清基质模型中，聚（4VP-co-TRIM）提供了良好的色氨酸和犬尿氨酸的回收率（76％和87％，分别)，并允许减少基体效应。将两种共聚物的性能与市售吸附剂（十八烷基硅烷，活性炭,和伯仲胺)。
