Abstract：
Endometritis and metritis are common reproductive diseases in domestic animals, causing a reduction in reproductive performance and economic losses. A previous study revealed the alterations in the transcriptome of the inflamed porcine endometrium. Data on molecular signatures in the myometrium under inflammatory conditions are limited. The current study analyzed the transcriptomic profile of porcine myometrium after intrauterine Escherichia coli (E.coli) administration. On day 3 of the estrous cycle (Day 0 of the study), 50 ml of either saline (group CON, n = 7) or E. coli suspension (109 colony-forming units/ml, group E. coli, n = 5) were injected into each uterine horn. After eight days, the gilts were euthanized, and the uteri were removed for further analysis. In the myometrium of the CON group versus the E. coli group, microarray analysis revealed 167 differentially expressed genes (DEGs, 78 up- and 89 down-regulated). After intrauterine E. coli administration, among the DEGs of the inflammatory response set, the highest expressed were mRNA for CXCL6, S100A8, S100A12, SLC11A1, S100A9, CCL15, CCR1, CD163, THBS1 and SOCS3, while the most suppressed was mRNA expression for FFAR4, KL, SLC7A2 and MOAB. Furthermore, a comparison of the present results on myometrial transcriptome with the authors\' earlier published data on the endometrial transcriptome shows the partial differences in mRNA expression between both layers after intrauterine E.coli injections. This study, for the first time, presents changes in the transcriptome of porcine myometrium after intrauterine E.coli administration, which may be important for myometrial homeostasis and functions and, as a result, for the uterine inflammation course. Data provide a valuable resource for further studies on genes and pathways regulating uterine inflammation and functions.
摘要：
子宫内膜炎和子宫炎是家畜常见的生殖疾病，造成生殖性能下降和经济损失。先前的研究揭示了发炎的猪子宫内膜的转录组的改变。炎症条件下子宫肌层的分子特征数据有限。当前的研究分析了子宫内大肠杆菌（E.大肠杆菌)管理。在发情周期的第3天（研究的第0天），50ml的任一生理盐水（CON组，n=7）或大肠杆菌悬浮液（109菌落形成单位/ml，大肠杆菌组，n=5）被注射到每个子宫角中。八天后,母猪被安乐死，并取出子宫用于进一步分析。在CON组与大肠杆菌组的子宫肌层中，微阵列分析显示167个差异表达基因(DEGs，78上调，89下调）。在子宫内施用大肠杆菌后，在炎症反应组的DEG中，表达最高的是CXCL6，S100A8，S100A12，SLC11A1，S100A9，CCL15，CCR1，CD163，THBS1和SOCS3的mRNA表达，而抑制最多的是FFAR4，KL，SLC7A2和MOAB。此外，对目前子宫肌层转录组的结果与作者早期发表的子宫内膜转录组数据的比较显示,在子宫内注射大肠杆菌后,两层之间的mRNA表达存在部分差异.这项研究,第一次,介绍了子宫内施用大肠杆菌后猪子宫肌层转录组的变化，这对子宫肌层稳态和功能可能很重要，因此,子宫发炎的过程。数据为进一步研究调节子宫炎症和功能的基因和途径提供了宝贵的资源。
