Abstract：
Schistosomiasis caused by Schistosoma japonicum (S. japonicum) is a major public health problem in the Philippines, China and Indonesia. In this study, the immunopotentiator CpG-ODN was encapsulated within chitosan nanoparticles (Chi NPs) to create a combination adjuvant (Chi-CpG NP). This approach was employed to enhance the immunogenicity of 26 kDa glutathione S-transferase (Sj26GST) from S. japonicum through intranasal immunization. The results demonstrated higher levels of specific anti-Sj26GST antibodies and Sj26GST-specific splenocyte proliferation compared to mice that were immunized with Sj26GST + Chi-CpG NP. Cytokine analysis of splenocytes revealed that the Sj26GST + Chi-CpG NP induced a slight Th1-biased immune response, with increased production of IFN-γ by CD4+ T-cells in the spleen. Subsequently, mice were intradermally inoculated with 1 × 107 organisms in the Coeliac cavity. The bacterial organ burden detected in the liver of immunized mice suggested that Sj26GST + Chi-CpG NP enhances protective immunity to inhibit S. japonicum colonization. Therefore, Sj26GST + Chi-CpG NP vaccination enhances Sj26GST-specific immunogenicity and provides protection against S. japonicum.
摘要：
日本血吸虫引起的血吸虫病（S.japonicum）是菲律宾的主要公共卫生问题，中国和印度尼西亚。在这项研究中,将免疫增强剂CpG-ODN包封在壳聚糖纳米颗粒(ChiNP)中以产生组合佐剂(Chi-CpGNP)。该方法用于通过鼻内免疫增强来自日本血吸虫的26kDa谷胱甘肽S-转移酶(Sj26GST)的免疫原性。结果显示,与用Sj26GST+Chi-CpGNP免疫的小鼠相比,更高水平的特异性抗Sj26GST抗体和Sj26GST特异性脾细胞增殖。脾细胞的细胞因子分析显示，Sj26GST+Chi-CpGNP诱导了轻微的Th1偏向性免疫应答，脾脏中CD4+T细胞产生的IFN-γ增加。随后，小鼠腹腔皮内接种1×107种生物。在免疫小鼠的肝脏中检测到的细菌器官负荷表明Sj26GSTChi-CpGNP增强保护性免疫以抑制日本血吸虫定植。因此,Sj26GST+Chi-CpGNP疫苗接种增强Sj26GST特异性免疫原性并提供针对日本血吸虫的保护。
